COSMOSIL πNAP is a reversed phase HPLC column with Naphtylethyl group bonded silica packing material. The naphthylethyl group is composed of two fused aromatic rings and forms strong π-π interactions with unsaturated compounds. This column offers improved separation of compounds such as positional isomers that are difficult to analyze with alkyl group bonded materi-als.

HPLC Column for Structural Isomers

α(Nitrobenzene/Benzene)1.00 1.20 1.40 1.60

Ph

PE-MS

πNAP

π-π Interaction

Ph

SiCH3H3C

πNAP

SiCH3H3C

Comparison with Phenyl Columns COSMOSIL πNAP with two aromatic rings has stronger π-π interactions than phenyl column which has one aromatic ring.

The Interaction between Benzene and Nitrobenzene

SiCH3H3C

PE-MS

Stationary Phase

Ph, PE-MSWeak π-π Interaction

C C

C C

C CH H

HH

H H

πNAPStrong π-π Interaction

C C

C C

C CH

H

H H

C

C CC H

HH

H

Sample

Benzene

Weak

Nitrobenzene

NO2

Strong

Naphtylethyl Group

● Naphthalene bonded stationary phase● Enhanced π-π interactions　● Improved selectivity for structural isomers

High Purity Porous Spherical Silica

5 μmapprox. 120Å

Naphtylethyl Group

Hydrophobic Interaction,

π-π Interactionapprox. 11%

Silica Gel

Average Particle Size

Average Pore Size

Stationary Phase

Main Interaction

Carbon Content

SiH3C CH3

5C18-MS-II(95% Methanol)

πNAP(90% Methanol)

5PE-MS(90% Methanol)

5PE-MS(40% Methanol)

πNAP(50% Methanol)

o- m-

p-

5C18-MS-II(40% Methanol)

5PE-MS(40% Methanol)

o- m-

p-

πNAP(50% Methanol)

N

CH3

C

o-

CH3

CN

m-

CH3

CNp-

● Position Isomers of Phthalonitriles

● Tocopherols

5C18-MS-II(30% Methanol)

Application Data

Column SizeFlow RateTemperatureDetection

4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 254 nm　

Column SizeFlow RateTemperatureDetection

4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 254 nm

CN

CN

CN

CN

CN

CN

O

HOR1

R2

R3

R1

R2

R3

α

Me

Me

Me

β

Me

H

Me

γ

H

Me

Me

δ

H

H

Me

Column SizeFlow RateTemperatureDetectionSample

4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 295 nmTocopherol (α, β, γ, δ)(1.25 μg each)　　

● Position Isomers of Tolunitriles

Sample o-Tolunitrilem-Tolunitrilep-Tolunitrile 　　

(2.0 μg) (2.0 μg) (1.0 μg) 　

Sample o-, Phthalonitrilem-, Isophthalonitrilep-, Terephthalonitrile 　　

(0.3 μg) (3.0 μg) (0.15 μg)

o- m- p-

● Vitamin E

5C18-MS-II πNAP Column SizeMobile Phase

Flow RateTemperatureDetectionSample

4.6 mm I.D. x 250 mm5C18-MS-IIπNAP1.0 ml/min30℃UV 295 nm1. δ-Tocotrienol2. γ-Tocotrienol3. β-Tocotrienol4. α-Tocotrienol5. δ-Tocopherol6. γ-Tocopherol7. β-Tocopherol8. α-Tocopherol

: Methanol: Methanol : H2O = 90 : 10

5C18-MS-II πNAP 5PE-MS

5C18-MS-II 5PE-MS πNAP

5C18-MS-II(50% Methanol)

5PE-MS(50% Methanol)

πNAP(60% Methanol)

● Adrenal Cortical Hormones

● Isoflavones

● Berberines

HO

O

OOH

HOHO

O

OOH

HO OHO

O

OOH

1.Prednisolone 2.Hydrocortisone 3.Cortisone

N+MeO

OMe

OMe

OMe

N+MeO

OMe

O

O

Cl- Cl-

1. Palmatine Chloride 2. Berberine Hydrochloride

Column SizeFlow RateTemperatureDetectionSample

Column SizeMobile Phase

Flow RateTemperatureDetection

4.6 mm I.D. x 150 mmA: Acetonitrile : 20 mmol/l Phosphate Buffer(pH2.5) = 10 : 90B: Acetonitrile : 20 mmol/l Phosphate Buffer(pH2.5) = 50 : 50B　0→100%/20 min Linear Gradient1.0 ml/min30℃UV 260 nm

(0.05 μg)(0.05 μg)(0.05 μg)(0.05 μg)(0.075 μg)(0.025 μg)　　

Column SizeMobile Phase

Flow RateTemperatureDetection

4.6 mm I.D. x 150 mm5C18-MS-II : Methanol : 20 mmol/l Phosphate Buffer(pH2.5) = 40 : 605PE-MS : Methanol : 20 mmol/l Phosphate Buffer(pH2.5) = 60 : 40πNAP　　 : Methanol : 20 mmol/l Phosphate Buffer(pH2.5) = 80 : 201.0 ml/min30℃UV 254 nm

Sample1. Daidzin2. Glycitin3. Genistin4. Daidzein5. Glycitein6. Genistein

Sample 1. Palmatine Chloride 2. Berberine Hydrochloride (0.2 μg each) 　　

4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 254 nm1. Prednisolone2. Hydrocortisone3. Cortisone

(0.33 μg)(0.33 μg)(0.33 μg)

● Adrenal Cortical Hormones

5C18-MS-II πNAP

Column SizeMobile Phase

Flow Rate

4.6 mm I.D. x 150 mm5C18-MS-IIπNAP1.0 ml/min

TemperatureDetection

30℃UV 254 nm: Methanol : H2O = 50 : 50

: Methanol : H2O = 60 : 40

Sample 1. Triamcinolone2. Cortisone3. Prednisolone4. Hydrocortisone5. Betamethasone6. Dexamethasone7. Triamcinolone Acetonide8. Cortisone Acetate9. Prednisolone Acetate10. Fluocinolone Acetonide11. Fluorometholone(0.23 μg each)

NACALAI TESQUE, INC.Nijo Karasuma, Nakagyo-kuKyoto 604-0855, JAPANTELFAXWebsiteE-mail

For research use only, not intended for diagnostic or drug use.

1104

: +81-(0)75-251-1730: +81-(0)75-251-1763: http://www.nacalai.com: info.intl@nacalai.com

Further Separation

5C18-MS-II(98% Methanol)

πNAP(90% Methanol)

HO

R R =

2.Stigmasterol

1.Cholesterol

3.Campesterol

4.Sitosterol

COSMOSIL PYE is a packed column with pyrene bonded silica packing material. It has stronger π-π interaction than πNAP since pyrene has four aromatic rings.

5PYE(95% Methanol)

● Sterols

Column SizeFlow RateTemperatureDetectionSample

4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 210 nm1. Cholesterol2. Stigmasterol3. Campesterol4. Sitosterol 　　

Ordering Information

Product Number

08082-71

08087-21

08090-61

08091-51

08094-21

Product Name

COSMOSIL πNAP Guard Column

Column Size

4.6 mm I.D. x 10 mm

10.0 mm I.D. x 20 mm

20.0 mm I.D. x 20 mm

20.0 mm I.D. x 50 mm

28.0 mm I.D. x 50 mm

Other size may be available. Please enquire.

Product Name

COSMOSIL πNAP Packed Column

Column Size

1.0 mm I.D. x 150 mm

1.0 mm I.D. x 250 mm

2.0 mm I.D. x 30 mm

2.0 mm I.D. x 50 mm

2.0 mm I.D. x 100 mm

2.0 mm I.D. x 150 mm

2.0 mm I.D. x 250 mm

3.0 mm I.D. x 150 mm

3.0 mm I.D. x 250 mm

4.6 mm I.D. x 150 mm

4.6 mm I.D. x 250 mm

10.0 mm I.D. x 150 mm

10.0 mm I.D. x 250 mm

20.0 mm I.D. x 150 mm

20.0 mm I.D. x 250 mm

28.0 mm I.D. x 250 mm

Product Number

08076-61

08077-51

08566-41

08567-31

08299-51

08078-41

08079-31

08080-91

08081-81

08085-41

08086-31

08088-11

08089-01

08092-41

08093-31

08095-11

CH3Si

H3C

SiH3C CH3