Glykolyse - ein Stoffwechselweg zur schnellen Bereitstellung von Energie.
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Transcript of Glykolyse - ein Stoffwechselweg zur schnellen Bereitstellung von Energie.
Glykolyse- ein Stoffwechselweg zur
schnellen Bereitstellung von Energie
N
NN
N
NH2
O
OHOH
CH2O P O P O P
O
O
O
O
O
O
O
ATP
Adenin
Triphosphat
Ribose
Adenosin
Säureanhydridbindungen
2
α-D-Glucose
PAdenosin PP~ ~
O
CH2O
OH
OHHO OH
H PO32-
1
23
4
5
6
Glucose-6-phosphat
Glykolyse: Stufe 1
3
α-D-GlucoseO
CH2OH
OH
OHHO OH
Glucose-6-phosphatO
CH2O
OH
OHHO OH
Hexokinase
ATP
P
Glykolyse: Stufe 1 Phosphorylierung
ADP
4
O
OCH2
OH
OHHO OH
H
H
Glucose-6-phosphat
Fructose-6-phosphat
Glucose-6-phosphat-isomerase
P
C
C
O H
C
C
C
CH2O
OH
HO
OH
OH
H
H
H
H
CO
C
C
C
CH2O
HO
OH
OH
H
H
H
CH2OH
Glykolyse: Stufe 1 Isomerisierung
α-D-GlucoseATP
5
CO
C
C
C
CH2O
HO
OH
OH
H
H
H
CH2O
Fructose-6-phosphat
Fructose-1,6-bisphosphat
Phosphofructo-kinase
ATP
CO
C
C
C
CH2O
HO
OH
OH
H
H
H
CH2OH
Glykolyse: Stufe 1 Phosphorylierung
ADP
α-D-GlucoseATP
6
CO
C
C
C
CH2O
HO
OH
OH
H
H
H
CH2O
Fructose-1,6-bisphosphat
C
C
H O
OHH
CH2O
Dihydroxy-acetonphosphat
Glycerinaldehyd-3-phosphat
Aldolase
+
Glykolyse: Stufe 2 Aldolspaltung
CO
CHO H
CH2O
H
DHAP GAP
α-D-GlucoseATP
ATP
Fructose-1,6-bisphosphat
7
CO
C
C
C
CH2O
HO
OH
OH
H
H
H
CH2O
Fructose-1,6-bisphosphat
C
C
H O
OHH
CH2O
Dihydroxy-acetonphosphat
Glycerinaldehyd-3-phosphat
Aldolase
Glykolyse: Stufe 2 Aldolspaltung
CO
CHO H
CH2O
H
α-D-GlucoseATP
ATP
Triosephosphat-isomerase
GAPDHAP
Fructose-1,6-bisphosphat
8
Glycerinaldehyd-3-phosphat2
1,3-Bisphosphoglycerat2
Glycerin-aldehyd-
3-phosphat-Dehydrogenase
C OHH
CH2O
C O H
C OHH
CH2O
C O H
C OHH
CH2O
C O O
C OHH
CH2O
C O O
Glykolyse: Stufe 3 Oxidation u. Phosphorylierung
NAD+, Pi
P
O
O
OH
O
NAD+, Pi
NADH NADH
α-D-GlucoseATP
ATP
Fructose-1,6-bisphosphat
GAPDHAP
9
21,3-Bisphosphoglycerat
2
Phospho-glycerat-kinase
3-Phosphoglycerat
OOC
C OHH
CH2O
OOC
C OHH
CH2O
C OHH
CH2O
C O O
C OHH
CH2O
C O O
Glykolyse: Stufe 3 Substratkettenphosphorylierung
ADP ADP
ATP ATP
α-D-GlucoseATP
ATP
Fructose-1,6-bisphosphat
GAPDHAP
NADH NADH
10
2
2
Phospho-glycerat-mutase
2-Phosphoglycerat
OOC
C OHH
CH2O
OOC
C OHH
CH2O
3-Phosphoglycerat
C
C OH
CH2OH
O O
C
C OH
CH2OH
O O
Glykolyse: Stufe 3 Phosphatverschiebung
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH NADHATP ATP
11
2
Enolase
Phosphoenol-pyruvat
C
C OH
CH2OH
O O
C
C OH
CH2OH
O O
2-Phosphoglycerat
O
O CC
O
CHH
O
O CC
O
CHH
Glykolyse: Stufe 3 Wasserabspaltung
H2O
2
H2O
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH NADHATP ATP
PEP PEP
12
2
2
Pyruvatkinase
Pyruvat (Enolform)
Phosphoenol-pyruvat
O
O CC
OH
CHH
O
O CC
OH
CHH
O
O CC
O
CHH
O
O CC
O
CHH
Glykolyse: Stufe 3 ATP-Bildung
ADP ADP
ATP ATP
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH NADHATP
PEP
Pyruvat
PEP
Pyruvat
ATP
13
2
2
Pyruvat (Enolform)
O
O CC
OH
CHH
O
O CC
OH
CHH
Pyruvat (Ketoform)C
CO
CH3
O
O
CC
O
CH3
O
O
Glykolyse: Stufe 3
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH NADHATP
PEP
Pyruvat
PEP
Pyruvat
ATP
ATP ATP
14
Glycerinaldehyd-3-phosphat2
1,3 Bisphosphoglycerat2
C OHH
CH2O
C O H
C OHH
CH2O
C O H
C OHH
CH2O
C O O
C OHH
CH2O
C O O
Glykolyse: Stufe 3
NADH
NAD+
NADH
NAD+, Pi , Pi
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADHATP
PEP
Pyruvat
PEP
Pyruvat
ATPNADH
ATP ATP
15
C OHH
CH2O
C O H NAD+
H2O
C OHH
CH2O
C O OH
Glykolyse: Stufe 3
NADH
H+
Oxidation
+
+1 +3
C OHH
CH2O
C O OH
1.
2.P
O
O
OH
O
C OHH
CH2O
C O O
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH NADH
16
C OHH
CH2O
C HO
R
HS
Glykolyse: Stufe 3 – katalytischer Mechanismus
Thiohalbacetal
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
17
CONH2
N RH NH R
CONH2
C O H
RS
H
Histidin NAD+
Glykolyse: Stufe 3 – katalytischer Mechanismus
Thioester-Zwischenprodukt
NADHα-D-Glucose
ATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
18
N RH
CONH2
C O
RS
Histidin NAD+
Glykolyse: Stufe 3 – katalytischer Mechanismus
H
Thioester-Zwischenprodukt
P
O
O
OH
O
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH
19
C OHH
CH2O
C O O
C O
R S
Histidin NAD+
Glykolyse: Stufe 3 – katalytischer Mechanismus
H O
1,3 Bisphosphoglycerat
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH
20
N RH
CONH2
Glykolyse: Zusammenfassung
O
CH2OH
OH
OHHO OH
CC
O
CH3
O
O
CC
O
CH3
O
OATP ATP
NADH NADH
Lactat
NAD+
NAD+
O
O CC
CH3
OH
O
O CC
CH3
OH
α-D-GlucoseATP
GAPDHAP
ATP
Fructose-1,6-bisphosphat
NADH NADHATP
PEP
Pyruvat
PEP
Pyruvat
ATP
ATP ATP
21
