GigaMune TELS Product Sheet
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GigaMune® TELS™ Frustrated with current T cell assays? Take control with a single assay that gives you all the information you need. Discover which individual T cells express FOXP3, TNF-α, or other effector genes—without staining or cell sorting. Starting with a single-cell suspension, GigaMune® TELS™ ( TCR β - Effector Linkage Sequencing) uses unique microfluidics and molecular techniques to physically link T cell variable regions to effector genes expressed within millions of individual T cells in a sample. By sequencing TCRβ-effector linkage products, GigaMune® TELS™ gives you simultaneous, quantitative measurements of T cell clonal identity and effector gene activity. Never has a single assay combined accurate measures of T cell identity, clonality, expansion, and function. Let GigaMune® TELS™ revolutionize your understanding of the T cell response. T cell identity, function, and abundance in a single assay. IMMUNOPHENOTYPING APPLICATIONS Autoimmune diseases Transplant rejection and tolerance Immune response to malignancy Cancer immunotherapy Adoptive T cell therapy Asthma and allergy Inflammatory conditions Infectious diseases Vaccine development and efficacy Global immune function and health • • • • • • • • • • HIGHLIGHTS Millions of single cells processed per hour Detect rare and ultra-rare T cell clones from peripheral blood FEATURES BENEFITS Massively parallel amplification of transcripts produced by single cells Classify T cells based on expression of functional effector genes such as transcription factors Multiplexed measurements of TCRβ CDR3 sequence and effector gene transcripts Learn clonal identity, abundance, and function from the same sample High-quality sequence of TCRβ CDR3 regions Quantify and distinguish multiple clonotypes with specific effector functions Accurate measurements of clonal abundance Make objective, quantitative comparisons of clonal expansion across samples Powerful web-based software suite for data storage, processing, and analysis in the cloud Track abundance and expression profiles of clones across samples, with statistical analysis A REVOLUTION IN FUNCTIONAL T CELL ANALYSIS GigaGen laboratory and professional services Get reproducible results quickly with no startup costs or training time required GigaMune® TELS™ is For Research Use Only. Not for use in diagnostic procedures.
description
Product marketing sheet for GigaGen's innovative TELS platform for obtaining clonotype and immunophenotype of individual immune cells at high throughput.
Transcript of GigaMune TELS Product Sheet
GigaMune® TELS™
Frustrated with current T cell assays? Take control with a single assay that gives you all the information you need. Discover which individual T cells express FOXP3, TNF-α, or other effector genes—without staining or cell sorting. Starting with a single-cell suspension, GigaMune® TELS™ (TCRβ-Effector Linkage Sequencing) uses unique microfluidics and molecular techniques to physically link T cell variable regions to effector genes expressed within millions of individual T cells in a sample. By sequencing TCRβ-effector linkage products, GigaMune® TELS™ gives you simultaneous, quantitative measurements of T cell clonal identity and effector gene activity. Never has a single assay combined accurate measures of T cell identity, clonality, expansion, and function. Let GigaMune® TELS™ revolutionize your understanding of the T cell response.
T cell identity, function, and abundancein a single assay.
IMMUNOPHENOTYPING APPLICATIONS
Autoimmune diseases
Transplant rejection and tolerance
Immune response to malignancy
Cancer immunotherapy
Adoptive T cell therapy
Asthma and allergy
Inflammatory conditions
Infectious diseases
Vaccine development and efficacy
Global immune function and health
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HIGHLIGHTS
Millions of single cells processed per hour Detect rare and ultra-rare T cell clones from peripheral blood
FEATURES BENEFITS
Massively parallel amplification of transcripts produced by single cells
Classify T cells based on expression of functional effector genes such as transcription factors
Multiplexed measurements of TCRβ CDR3 sequence and effector gene transcripts
Learn clonal identity, abundance, and function from the same sample
High-quality sequence of TCRβ CDR3 regions Quantify and distinguish multiple clonotypes with specific effector functions
Accurate measurements of clonal abundance Make objective, quantitative comparisons of clonal expansion across samples
Powerful web-based software suite for data storage, processing, and analysis in the cloud
Track abundance and expression profiles of clones across samples, with statistical analysis
A REVOLUTION IN FUNCTIONAL T CELL ANALYSIS
GigaGen laboratory and professional services Get reproducible results quickly with no startup costs or training time required
GigaMune® TELS™ is For Research Use Only. Not for use in diagnostic procedures.
Copyright © 2012 GigaGen, Inc. All rights reserved.
Corporate Headquarters
409 Illinois Street
San Francisco, CA 94158
www.gigagen.com
phone: 415.409.8751
fax: 415.418.3494
email: [email protected]
GigaMune® TELS™ 120914
Want to learn more? Email us at [email protected] to have a representative contact you.
View data using ClonoByte™ web-based software for data storage,
processing, statistical analysis, sample comparison, and data
visualization in the cloud.
How GigaMune® TELS™ Works
1 Make single-cell suspension.
Encapsulate single cells.
Link TCRβ cDNA with effector gene cDNA.
Sequence TCRβ-effector linkage products.
Analyze results.
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Tissue is dissociated into a single-cell suspension. Acceptable
tissues include peripheral blood and organ biopsies.
Pre-sorting by flow cytometry or magnetic beads is optional.
Cells are loaded onto a microfluidic device and individually
isolated in picoliter microdroplets at a rate of hundreds of cells
per second.
Single cells are lysed within microdroplets, and reagents and
multiplexed primers are delivered. cDNA is synthesized from
rearranged TCRβ genes and effector genes, followed by physical
linkage of TCRβ cDNA and effector cDNA. These processes all
occur within microdroplets, so that linked cDNA originates from an
individual cell.
Microdroplets are coalesced together and linkage products are
purified. Tags are added to linkage products in preparation for
massively parallel sequencing of TCRβ CDR3 regions linked to
effector genes expressed by the same T cell clone.
FOXP3
TCRβ
