Galactose conversion
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Transcript of Galactose conversion
Galactose Conversion
GROUP IIIImanuelle OrchideaNugroho Windu HadiRohsi Purnama Sari
Before Glycolisis
Galactose Structure in Comparison with Glucose
Lactose Hydrolisis
How galactose change into
glucose?
1. Conversion of β-D-galactose to α-D-galactose
2. Phosphorylation on C1 with ATP and galactokinase
ATP + D galactose -----> D galactose-1-phosphate+ ADP + H
Mg2+
+
Ribbon representation of galactose mutarotase from L. lactis.
The two subunits of the dimeric protein are displayed in red and blue. Bound galactose molecules are depicted in ball-and-stick representations. X-ray coordinates were from Protein Data Bank number 1L7K.
Cartoon diagram of a human galactokinase 1 monomer in complex with galactose (red) and an analogue of adenosine triphosphate (orange).A magnesium ion is visible as a green sphere.
3. Conversion of galactose-1-phosphate into glucose-1-phosphate by galactose-1-phosphate uridyltransferase
Ribbon representation of galactose-1-phosphate uridylyltransferase from E. coli.
The two subunits of the dimeric enzyme are displayed in red and blue with the positions of the metals indicated by the round spheres. The active site in this protein model contains bound UDP-glucose. X-ray coordinates were from Protein Data Bank number 1GUQ.
Recycle of the UDP-galactose to UDP-glucose for the transferase reaction.
Ribbon representation of human UDP-galactose 4-epimerase.
Each subunit of the dimeric enzyme folds into two domains. The N- and C-terminal domains are color-coded in blue and red, respectively. Bound NADH and UDP-glucose molecules are drawn in ball-and-stick representations. X-ray coordinates were from Protein Data Bank number 1EK6.