Free Powerpoint TemplatesPage 1Free Powerpoint Templates Modification of cytokinin level in Hordeum...
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Free Powerpoint Templates Page 1 Free Powerpoint Templates Modification of Modification of cytokinin level cytokinin level in in Hordeum vulgare Hordeum vulgare Centre of the Region Haná for Biotechnological and Agricultural Research Mgr.Katarína Mrízová Mgr.Hana Pospíšilová, Ph.D
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Transcript of Free Powerpoint TemplatesPage 1Free Powerpoint Templates Modification of cytokinin level in Hordeum...
Free Powerpoint Templates Page 1Free Powerpoint Templates
Modification of Modification of cytokinin level incytokinin level in Hordeum vulgareHordeum vulgare
Centre of the Region Haná for Biotechnological and Agricultural Research
Mgr.Katarína Mrízová Mgr.Hana Pospíšilová, Ph.D
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Constructs for barley transformationConstructs for barley transformation
Promoter Gene Targeting
β-GLU ZmCKX1 secreted
PHT ZmCKX1 secreted
RAF ZmCKX1 secreted
β-GLU AtCKX1 vacuolar
β-GLU AtCKX1 cytosolic
β-GLU AtCKX1 secreted
β-GLUAtCKX1 plastidic
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Root specific promotersRoot specific promoters
Control
Promoter of root abundant protein (RAF; hypotetical transcription factor)
Promoter of phosphate transporter (PHT)
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Root specific promotersRoot specific promoters
Promoter of CK-specific β-glucosidase (β-GLU)
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Results I.Results I.
Construct β-GLU::ZmCKX1 PHT::ZmCKX1 RAF::ZmCKX1
Transform. efficiency %
7 4 4
Relative expression
level of transgene in
roots
170-6331 17766 46-6645
CKX activity roots
up to 10-fold up to 2-fold up to 2-fold
Relative expression
level of transgene in
leaves
22856 1256-18051
CKX activity leaves
up to 5-fold up to 122-fold up to 101-fold
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PHT WT PHT WT
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WT RAF1 RAF2
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WT β-GLU1 β-GLU2
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Promoter Gene Targeting
β-GLUZmCKX1 secreted
PHTZmCKX1 secreted
RAFZmCKX1 secreted
β-GLUAtCKX1 vacuolar
β-GLUAtCKX1 cytosolic
β-GLUAtCKX1 secreted
β-GLUAtCKX1 plastidic
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Results II.Results II.
gene AtCKX1
Transform. efficiency %
Relative expression level of transgene in
roots
CKX activity roots
Generation
cytosolic 70 53-1000 up to 10-fold T1
vacuolar 18 278-8664 up to 7-fold T1
secreted 17 37-185 up to 10-fold T1
plastidic 70 57-847 up to 7-fold T1
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T2 generation of transgenic barley with vacuolar AtCKX1
WT Homozygot WT Heterozygot
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T2 generation of transgenic barley with vacuolar AtCKX1
WT Homozygot Heterozygot
