For Research Use Only NF-H Monoclonal ANTIBODY · 2020. 8. 31. · Selected Validation Data pig...
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NF-H Monoclonal ANTIBODY Catalog Number: 60331-1-Ig 1 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 60331-1-Ig Size: 150UL , Concentration: 1500 μg/ml by Bradford method using BSA as the standard; Source: Mouse Isotype: IgG2a Immunogen Catalog Number: AG13517 GenBank Accession Number: BC014185 GeneID (NCBI): 4744 Full Name: neurofilament, heavy polypeptide Calculated MW: 112 kDa Observed MW: 200 kDa Purification Method: Protein A purification CloneNo.: 1A3C7 Recommended Dilutions: WB 1:1000-1:8000 IHC 1:20-1:200 IF 1:20-1:200 Applications Tested Applications: IF, IHC, WB, ELISA Cited Applications: IHC Species Specificity: human, pig Cited Species: mouse Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : pig brain tissue, IHC : human brain tissue, IF : SH-SY5Y cells, Background Information NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only. Notable Publications Author Pubmed ID Journal Application Huangao Zhou 32474063 J Chem Neuroanat IHC Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of For Research Use Only NF-H Monoclonal ANTIBODY · 2020. 8. 31. · Selected Validation Data pig...
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NF-H Monoclonal ANTIBODYCatalog Number:60331-1-Ig 1 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:60331-1-Ig
Size:150UL , Concentration: 1500 μg/ml byBradford method using BSA as thestandard;
Source:Mouse
Isotype:IgG2a
Immunogen Catalog Number:AG13517
GenBank Accession Number:BC014185
GeneID (NCBI):4744
Full Name:neurofilament, heavy polypeptide
Calculated MW:112 kDa
Observed MW:200 kDa
Purification Method:Protein A purification
CloneNo.:1A3C7
Recommended Dilutions:WB 1:1000-1:8000 IHC 1:20-1:200 IF 1:20-1:200
Applications Tested Applications:IF, IHC, WB, ELISA
Cited Applications:IHC
Species Specificity:human, pig
Cited Species:mouse
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : pig brain tissue,
IHC : human brain tissue,
IF : SH-SY5Y cells,
Background Information NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important functionin mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediatefilaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide supportfor normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and Hwhich are involved in the maintenance of neuronal caliber. The names given to the three major neurofilamentsubunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa;NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.
Notable Publications Author Pubmed ID Journal ApplicationHuangao Zhou 32474063 J Chem Neuroanat IHC
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
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Selected Validation Data
pig brain tissue were subjected to SDS PAGEfollowed by western blot with 60331-1-Ig (NF200Antibody) at dilution of 1:4000 incubated at roomtemperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human brain using 60331-1-Ig(NF200antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain using 60331-1-Ig(NF200antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of SH-SY5Y cells using60331-1-Ig (NF200 antibody) at dilution of 1:50 andAlexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
