CCR2a-specific PolyclonalantibodyCatalog Number:16153-1-AP 3 Publications

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:16153-1-AP

Size:150UL , Concentration: 600 μg/ml byNanodrop and 373 μg/ml by Bradfordmethod using BSA as the standard;

Source:Rabbit

Isotype:IgG

GenBank Accession Number:NM_000647

GeneID (NCBI):729230

Full Name:chemokine (C-C motif) receptor 2

Calculated MW:42 kDa

Observed MW:42 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:500-1:1000 IHC 1:20-1:200

Applications Tested Applications:FC, IHC, WB,ELISA

Cited Applications:IF, IHC, WB

Species Specificity:human

Cited Species:human, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : Jurkat cells, A431 cells

IHC : human kidney tissue, human ovary tumor tissue

Background Information CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration and monocyte invasionof artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms wereexpressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and bysome mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression wasobserved in all satellite cells, in the muscular domain of neuromuscular junctions and in some regenerative fibersof IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. Thisantibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.

Notable Publications Author Pubmed ID Journal ApplicationHiroaki Yasui 31541326 Clin Exp Metastasis IHC

Huijie Zhuang 29115520 Oncol Rep WB

Hang Xian 31805255 Neuroscience IF

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Jurkat cells were subjected to SDS PAGE followedby western blot with 16153-1-AP (CCR2-specificantibody) at dilution of 1:200 incubated at roomtemperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney slide using 16153-1-AP(CCR2-specific antibody at dilution of 1:50.

Immunohistochemical analysis of paraffin-embedded human kidney slide using 16153-1-AP(CCR2-specific antibody at dilution of 1:50.

1X10^6 Jurkat cells were stained with 0.2ug CCR2-specific antibody (16153-1-AP, red) and controlantibody (blue). Fixed with 90% MeOH blockedwith 3% BSA (30 min). Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L) with dilution1:1000.