A Point-Distribution Index and Its Application to Sensor Grouping Problem
Fluorogenic Polydiacetylene Nanosomes: Application to ...
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1 Fluorogenic Polydiacetylene Nanosomes: Application to Label-Free Nanobio Sensors Dong June Ahn [email protected], +82-2-3290-3301 Department of Chemical & Biological Engineering College of Engineering, Korea University Seoul, Korea April 17-19, 2008 D. J. Ahn, Korea University Diagnoses & Drug Discovery Bio Chips & Chemical Chips Needs for Sensors Safety for Processed Food Anthrax New & Mutated Infectious Diseases Need for Disease Monitoring Astronomical cost spent each year worldwide ! SARS Mutated Cholera, etc. D. J. Ahn, Korea University
Transcript of Fluorogenic Polydiacetylene Nanosomes: Application to ...
Microsoft PowerPoint - Ahn_DJ_2008_.pptDong June Ahn
Seoul, Korea
Diagnoses & Drug Discovery
worldwide !
SARS
2
Outline
Current Concept for Sensing Systems and Chips
D. J. Ahn, Korea University
Target Substance Recognizing Materials Human Judgment
A
BC
A
A
A
Affinity Shape Size
Signal Transducer Transistors / Optics
Protein ChipDNA Chip
A G T
T C AT
target molecule
S
T
S-T
• π-Conjugated Polymer Sensor
• π-Conjugated Polymers
R R
R R
4
Ligand
O
X X X
O O O
conjugated p-orbital twisted p-orbital
6
increase of virus concentration
Detection of Influenza Virus A Charych et al., Science, 261, 585 (1993); J. Am. Chem. Soc., 117, 829 (1995).
D. J. Ahn, Korea University
OH NH
OH O
O O
pore match
D. J. Ahn, Korea University
7
Films on Substrates Method: Langmuir-Schaefer Use: Film-type Sensor Low Signal One Binding Event per Film
OH OH OH
O O O
Blue
Blue
Blue
Purple
Advanced Materials, 19, 521 (2007).
Surface Immobilized Nanosomes
Method: Microcontact Printing Use: Sensor
One Binding Event per Patterned Nanosome Surface
Method: Inkjet Spotting Use: Chemical & Biological Chip
Simultaneous Screening & Detection
D. J. Ahn, Korea University
Nanosome Solutions Method: Sonication Use: Solution-type Sensor Medium-High Signal One Binding Event per Solution
Nanosome-Based Chips
8
< Ink-jet Microarrayer >
Pre-spot Nanosome Library Solutions in 96 or 384 well plates
APS gl as
Inkjet (non-contact), single tip
1 spot (4 nL), width: 200 • Conventional sol’n analysis: 0.1 mL
• 25,000-fold saving
Polyacrylic acid (30mM)
D. J. Ahn, Korea University Ahn et. al., J. Am. Chem. Soc., 127, 17580 (2005).
9
after heated
when primary antibody is absent
after exposure to anti-mouse secondary antibodies
D. J. Ahn, Korea University
Advanced Functional Materials (2007); Macromolecular Research (2006).
A Label-Free Detection of Bacteria
D. J. Ahn, Korea University
10
when primary antibody is absent
D. J. Ahn, Korea University Submitted to Angew. Chem. Int. Ed. (2007).
DNA Detection Sensitivity
100 nM 10 nM 1 nM 100 pM 10 pM
1 pM 100 fM 10 fM 1 fM
Spotting of vesicle-probe DNA complex
Polymerization
5` - CTA CCT ACG GGT AAC - 3`
E. coli lethal factor sequence 15 mer.
D. J. Ahn, Korea University
100 nM 10 nM 1 nM 100 pM 10 pM 1 pM 100 fM 10 fM 1 fM -- 0
50
100
150
200
250
Polymerization
200
Vesicle
NH2 – 5` - GTT ACC CGT AGG TAG - 3`
3` - CAA TGG GCA TCC ATC - 5` TAG
A G T
12
Confocal Fluorescence Microscopic Image
13
after photopolymerization
hv
electrospinning
14
hairtail, croaker, skate, etc chickenbeef, pork
pH=8.8 pH=11.0 pH=12.3
Liquid Analytes Gas Analytes
HCHO detection
15
+ Target
- Target
Blue
Blue
Blue
Purple
Pink
Red
100
Summary of Sensor Research
Acknowledgments
Dr. D. H. Charych (Lawrence Berkeley Nat’l Lab. / Chiron Corp. / Five Prime Corp.) Prof. Jong-Man Kim (Hanyang University)
Research Funds from Korean Government (KISTEP, KRF, KOSEF, MOH) and Private Sectors
Home Coming Day
My Dear Students
Seoul, Korea
Diagnoses & Drug Discovery
worldwide !
SARS
2
Outline
Current Concept for Sensing Systems and Chips
D. J. Ahn, Korea University
Target Substance Recognizing Materials Human Judgment
A
BC
A
A
A
Affinity Shape Size
Signal Transducer Transistors / Optics
Protein ChipDNA Chip
A G T
T C AT
target molecule
S
T
S-T
• π-Conjugated Polymer Sensor
• π-Conjugated Polymers
R R
R R
4
Ligand
O
X X X
O O O
conjugated p-orbital twisted p-orbital
6
increase of virus concentration
Detection of Influenza Virus A Charych et al., Science, 261, 585 (1993); J. Am. Chem. Soc., 117, 829 (1995).
D. J. Ahn, Korea University
OH NH
OH O
O O
pore match
D. J. Ahn, Korea University
7
Films on Substrates Method: Langmuir-Schaefer Use: Film-type Sensor Low Signal One Binding Event per Film
OH OH OH
O O O
Blue
Blue
Blue
Purple
Advanced Materials, 19, 521 (2007).
Surface Immobilized Nanosomes
Method: Microcontact Printing Use: Sensor
One Binding Event per Patterned Nanosome Surface
Method: Inkjet Spotting Use: Chemical & Biological Chip
Simultaneous Screening & Detection
D. J. Ahn, Korea University
Nanosome Solutions Method: Sonication Use: Solution-type Sensor Medium-High Signal One Binding Event per Solution
Nanosome-Based Chips
8
< Ink-jet Microarrayer >
Pre-spot Nanosome Library Solutions in 96 or 384 well plates
APS gl as
Inkjet (non-contact), single tip
1 spot (4 nL), width: 200 • Conventional sol’n analysis: 0.1 mL
• 25,000-fold saving
Polyacrylic acid (30mM)
D. J. Ahn, Korea University Ahn et. al., J. Am. Chem. Soc., 127, 17580 (2005).
9
after heated
when primary antibody is absent
after exposure to anti-mouse secondary antibodies
D. J. Ahn, Korea University
Advanced Functional Materials (2007); Macromolecular Research (2006).
A Label-Free Detection of Bacteria
D. J. Ahn, Korea University
10
when primary antibody is absent
D. J. Ahn, Korea University Submitted to Angew. Chem. Int. Ed. (2007).
DNA Detection Sensitivity
100 nM 10 nM 1 nM 100 pM 10 pM
1 pM 100 fM 10 fM 1 fM
Spotting of vesicle-probe DNA complex
Polymerization
5` - CTA CCT ACG GGT AAC - 3`
E. coli lethal factor sequence 15 mer.
D. J. Ahn, Korea University
100 nM 10 nM 1 nM 100 pM 10 pM 1 pM 100 fM 10 fM 1 fM -- 0
50
100
150
200
250
Polymerization
200
Vesicle
NH2 – 5` - GTT ACC CGT AGG TAG - 3`
3` - CAA TGG GCA TCC ATC - 5` TAG
A G T
12
Confocal Fluorescence Microscopic Image
13
after photopolymerization
hv
electrospinning
14
hairtail, croaker, skate, etc chickenbeef, pork
pH=8.8 pH=11.0 pH=12.3
Liquid Analytes Gas Analytes
HCHO detection
15
+ Target
- Target
Blue
Blue
Blue
Purple
Pink
Red
100
Summary of Sensor Research
Acknowledgments
Dr. D. H. Charych (Lawrence Berkeley Nat’l Lab. / Chiron Corp. / Five Prime Corp.) Prof. Jong-Man Kim (Hanyang University)
Research Funds from Korean Government (KISTEP, KRF, KOSEF, MOH) and Private Sectors
Home Coming Day
My Dear Students
