The Reaction of Some Sodium Salts of Fatty Acids and Benzoic
FluoProbesAltman FP "Tetrazolium salts and formazans". Prog Histochem Cytochem 9 (3): 1–56 (1976)....
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FluoProbes ® FT-65939A MTT Product Description . Name : MTT, Ultra Pure Grade 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide Catalog Number : FP-65939A Structure : C18H16BrN5S Molecular Weight : MW= 414,32 Solubility: Water, DMSO Absorption : λabs = 550 nm Storage: +4°C Protect from light and moisture. Warm to room temperature before opening. Introduction MTT is a tetrazolium salt that is commonly used to detect reductive metabolism in cells for viability, proliferation and cytotoxicity assays. On reduction tetrazolium salts turn into deeply colored products that can be easily quantified by colorimetric measurement. MTT forms a purple formazan product that needs to be solubilized prior to quantification. MTT is particularly useful for HTS of antiviral and anticancer compounds and for the studies of cytokine effect on cell proliferation. Directions for use Protocol 1) Plate 500-10,000 cells in 200ul media per well in a 96 well plate. Leave 8 wells empty for blank controls. 2) Incubate (37°C, 5% CO 2 ) overnight to allow the cells to attach to the wells. 3) Add 2ul of drug of interest dissolved in DMSO to each well. Place on a shaking table, 150rpm for 5 minutes, to thoroughly mix the samples into the media. 4) Incubate (37°C, 5% CO 2 ) for 1-5 days to allow the drug to take effect. 5) Make 2ml or more of MTT solution per 96 well plate at 5mg/ml in PBS. Do not make a stock as MTT in solution is not stable long-term. 6) Add 20ul MTT solution to each well. Place on a shaking table, 150rpm for 5 minutes, to thoroughly mix the MTT into the media. 7) Incubate (37°C, 5% CO 2 ) for 1-5 hours to allow the MTT to be metabolized. 8) Dump off the media. (dry plate on paper towels to remove residue if necessary. P.1
Transcript of FluoProbesAltman FP "Tetrazolium salts and formazans". Prog Histochem Cytochem 9 (3): 1–56 (1976)....
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FluoProbes®FT-65939A
MTT
Product Description.Name : MTT, Ultra Pure Grade
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
Catalog Number : FP-65939A
Structure : C18H16BrN5SMolecular Weight : MW= 414,32Solubility: Water, DMSOAbsorption : λabs = 550 nm
Storage: +4°C Protect from light and moisture. Warm to room temperature before opening.
Introduction MTT is a tetrazolium salt that is commonly used to detect reductive metabolism in cells for viability, proliferation and cytotoxicity assays. On reduction tetrazolium salts turn into deeply colored products that can beeasily quantified by colorimetric measurement. MTT forms a purple formazan product that needs to be solubilized prior to quantification. MTT is particularly useful for HTS of antiviral and anticancer compounds andfor the studies of cytokine effect on cell proliferation.
Directions for use
Protocol
1) Plate 500-10,000 cells in 200ul media per well in a 96 well plate. Leave 8 wells empty for blank controls.
2) Incubate (37°C, 5% CO2) overnight to allow the cells to attach to the wells.3) Add 2ul of drug of interest dissolved in DMSO to each well. Place on a shaking table, 150rpm for 5
minutes, to thoroughly mix the samples into the media. 4) Incubate (37°C, 5% CO2) for 1-5 days to allow the drug to take effect.5) Make 2ml or more of MTT solution per 96 well plate at 5mg/ml in PBS. Do not make a stock as MTT
in solution is not stable long-term.6) Add 20ul MTT solution to each well. Place on a shaking table, 150rpm for 5 minutes, to thoroughly mix
the MTT into the media. 7) Incubate (37°C, 5% CO2) for 1-5 hours to allow the MTT to be metabolized.8) Dump off the media. (dry plate on paper towels to remove residue if necessary.
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FluoProbes®FT-65939A
9) Resuspend formazan (MTT metabolic product) in 200ul DMSO. Place on a shaking table, 150rpm for 5 minutes, to thoroughly mix the formazan into the solvent.
10) Read optical density at 560nm and subtract background at 670nm. Optical density should be directly correlated with cell quantity.
References 1. Altman FP "Tetrazolium salts and formazans". Prog Histochem Cytochem 9 (3): 1–56 (1976).2. Marshall NJ, et al . "A critical assessment of the use of microculture tetrazolium assays to measure cell growth
and function". Growth Regul. 5 (2): 69–84 (1995)3. Scudiero DA, et al. "Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in
culture using human and other tumor cell lines". Cancer Res. 48 (17): 4827–33 (1988) Article
Technical and scientific information
Related / associated products and documentsSee BioSciences Innovations catalogue and e-search tool.
XTT, FP-40936A WST-1, F98883 WST-8, 899650
INT, UP020611 UptiBlue viable cell counting reagent,
UP669412
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Disclaimer : Materials from FluoProbes® are sold for research use only, and are not intended for food, drug, household, or cosmetic use. FluoProbes® is not liable for any damage resulting from handling or contact with this product.
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FluoProbes®MTTProduct DescriptionMTT, Ultra Pure Grade3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
IntroductionDirections for useProtocolReferences
Technical and scientific informationRelated / associated products and documents
Ordering information
