Evolution of transcription factor families along the human lineage
Events at initiation of transcription
description
Transcript of Events at initiation of transcription
Events at initiation of
transcription
Abortive initiation assayLet R = RNA polymerase, P = promoter (closed), and Po= promoter (open)
R + P RP RPoATP + UTP*
ApUp*U
kf
kr
KB
Abortive transcripts
[ApUp*U]
time
lag
Measure kf and KB from lag time vs. 1/[R]
Lag time
Lag time in abortive initiation assay is inversely proportional to [R].
Lag time = KB kf
1kf
1x +1[R]
1[R]Y-intercept =
kf
1
Slope = KB kf
1
Effect of wild-type and pc mutant λ repressors on activity of PR & PRM
Effect of Operator Mutations on Transcriptional Control of PR&PRM
OR1-OR2+OR3- OR1+OR2-OR3+
Effect of λ-pc mutations on KB and k2
Architecture of λOR
Mutations in the Activating Region ofλ Repressor
Glu
Glu
Glu
Mutations in the δ subunit of RNA polymerase that interfere w/λ repressor-mediated activation of PRM transcription
Effect of mutations in the δ subunit of RNA polymerase on activator-dependent and independent transcription of the lac
promoter
A model for interaction of the δ subunit of RNA polymerase with λ repressor
The Awesome Power of Genetics