ER Stress pathway - Abcamdocs.abcam.com/pdf/rabmab/er-stress-pathway.pdf · ER Stress pathway...

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Apoptosis Apoptos poptosis ATF6 (p50) ATF6 (p90) ATF6 (p90) PERK IRE1 IRE1 XBP1 mRNA splicing ATF6 (p50) Mitochondria Endoplasmic Reticulum Golgi Apparatus Cytochrome C UPR genes (GRP78, PDI, GRP94, etc) Secretion ROS Proteasomal Degradation ERSEs Unfolded Protein Nucleus Pro Caspase 12 Caspase 12 Caspase 3 Bcl-2 JNK TRAF2 ATF4 PP1 PP1 PP1 GADD34 XBP1s EDEM Derlin elF-2α CHOP ATF4 E ER R XBP1s ASK1 P P P P P Key Kinase Phosphatase Transcription Factor Ligand Translation ER Stress pathway Discover more at abcam.com/erstress

Transcript of ER Stress pathway - Abcamdocs.abcam.com/pdf/rabmab/er-stress-pathway.pdf · ER Stress pathway...

ApoptosisAApoptospoptosiiss

ATF6(p50)

ATF6(p90)

ATF6(p90)

PERKIRE1 IRE1

XBP1 mRNA splicing

ATF6(p50)

Mitochondria

EndoplasmicReticulum

Golgi Apparatus

Cytochrome C

UPR genes (GRP78, PDI, GRP94, etc)

Secretion

ROS Proteasomal Degradation

ERSEs

Unfolded Protein

Nucleus

Pro Caspase 12

Caspase 12Caspase 3

Bcl-2 JNK

TRAF2

ATF4

PP1PP1PP1

GADD34 XBP1s

EDEMDerlin

elF-2α

CHOPATF4

EERRRXBP1s

ASK1

PP P PP

Key

Kinase

Phosphatase

TranscriptionFactor

Ligand

Translation

ER Stress pathway

Discover more at abcam.com/erstress

Copyright © 2013 Abcam. All Rights Reserved.Discover more at abcam.com/erstress

ER Stress pathway

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab45178 at 1/10 dilution.

ASK1 (MEKK5) antibody [EP553Y] (ab45178)

star reviewed: Good

ASK1 is a component of a protein kinase signal transduction cascade. It phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases respectively.

ClonalityMApplicationFlow Cyt, ICC/IF, IHC-P, WBHostRb

Species reactivityHu, Ms

Antibody Clonality Application Host Species reactivity Product code

Bcl2 antibody [E17] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms ab32124Caspase 3 antibody [E87] M ICC/IF, IHC-P, IP, WB Rb Hu, Rt ab32351Cytochrome C antibody [EPR1327] M ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab133504

EIF2S1 (phospho S51) antibody [E90] M Dot Blot, ICC/IF, IHC-Fr, IHC-P, WB Rb Hu, Ms, Rt, Dm,

AGMk, Ncra ab32157

GRP78 BiP antibody [EPR4040(2)] M IHC-P, WB Rb Hu, Ms, Rt ab108613GRP94 antibody [EPR3988] M ICC/IF, IHC-P, WB Rb Hu, Ms ab108606IRE1 (phospho S724) antibody [EPR5253] M WB Rb Hu ab124945JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] M ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab124956

PPP1A antibody [EP1511Y] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab52619TRAF2 antibody [EPR6048] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab126758XBP1 antibody [EPR4086] M Flow Cyt, ICC/IF, IHC-P, WB Rb Hu ab109221

Related antibodies

Assay kit Sample type Product code

CytoPainter ER Staining Kit - Green Fluorescence Adherent cells, suspension cells ab139481CytoPainter ER Staining Kit - Red Fluorescence Adherent cells, suspension cells ab139482

Related assay kits

Substance Description Product code

SP600125 JNK inhibitor ab120065TCS JNK 5a Selective JNK2/3 inhibitor ab120835Z-D(OMe)E(OMe)VD(OMe)-FMK Cell permeable caspase-3 inhibitor ab120488Z-VAD(OMe)-FMK Cell permeable, irreversible pan-caspase inhibitor ab120487Z-VAD-FMK Irreversible general caspase inhibitor ab120382

Related biochemicals

Protein Expression system Applications Product code

ATF6 protein (Tagged) Wheat germ ELISA, SDS-PAGE, WB ab131846Caspase 3 peptide Blocking ab38283

NFAT5 protein Wheat germ ELISA, SDS-PAGE, WB, other ab112396

PERK protein (Active) E. coli Functional Studies, SDS-PAGE, WB ab101115

Related proteins

Why choose Alexa Fluor® conjugated secondaries from Abcam?

• Tested extensively in our laboratories - guarantee bright staining and low background• Large range of pre-adsorbed antibodies - ensure low species cross-reactivity• Dilution range of 1/200 -1/1000 (10-2 µg/ml) in ICC/IF - perform at least 250 stainings• Antibodies conjugated to Alexa Fluor®488/550/594/647 - for your multi-color imaging experiments

Secondary antibodies

Key: AGMk - African Green Monkey, Dm - Fruit fly (Drosophila melanogaster), Hu - Human, Ms - Mouse, Ncra - Neurospora crassa, Rb - Rabbit, Rt - Rat

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

EX

T_002_13

Product

highlights

- Rabbit Monoclonal Antibodies (RabMAbs)