ER Stress pathway - Abcamdocs.abcam.com/pdf/rabmab/er-stress-pathway.pdf · ER Stress pathway...
Transcript of ER Stress pathway - Abcamdocs.abcam.com/pdf/rabmab/er-stress-pathway.pdf · ER Stress pathway...
ApoptosisAApoptospoptosiiss
ATF6(p50)
ATF6(p90)
ATF6(p90)
PERKIRE1 IRE1
XBP1 mRNA splicing
ATF6(p50)
Mitochondria
EndoplasmicReticulum
Golgi Apparatus
Cytochrome C
UPR genes (GRP78, PDI, GRP94, etc)
Secretion
ROS Proteasomal Degradation
ERSEs
Unfolded Protein
Nucleus
Pro Caspase 12
Caspase 12Caspase 3
Bcl-2 JNK
TRAF2
ATF4
PP1PP1PP1
GADD34 XBP1s
EDEMDerlin
elF-2α
CHOPATF4
EERRRXBP1s
ASK1
PP P PP
Key
Kinase
Phosphatase
TranscriptionFactor
Ligand
Translation
ER Stress pathway
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ER Stress pathway
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab45178 at 1/10 dilution.
ASK1 (MEKK5) antibody [EP553Y] (ab45178)
star reviewed: Good
ASK1 is a component of a protein kinase signal transduction cascade. It phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases respectively.
ClonalityMApplicationFlow Cyt, ICC/IF, IHC-P, WBHostRb
Species reactivityHu, Ms
Antibody Clonality Application Host Species reactivity Product code
Bcl2 antibody [E17] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms ab32124Caspase 3 antibody [E87] M ICC/IF, IHC-P, IP, WB Rb Hu, Rt ab32351Cytochrome C antibody [EPR1327] M ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab133504
EIF2S1 (phospho S51) antibody [E90] M Dot Blot, ICC/IF, IHC-Fr, IHC-P, WB Rb Hu, Ms, Rt, Dm,
AGMk, Ncra ab32157
GRP78 BiP antibody [EPR4040(2)] M IHC-P, WB Rb Hu, Ms, Rt ab108613GRP94 antibody [EPR3988] M ICC/IF, IHC-P, WB Rb Hu, Ms ab108606IRE1 (phospho S724) antibody [EPR5253] M WB Rb Hu ab124945JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] M ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab124956
PPP1A antibody [EP1511Y] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab52619TRAF2 antibody [EPR6048] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rt ab126758XBP1 antibody [EPR4086] M Flow Cyt, ICC/IF, IHC-P, WB Rb Hu ab109221
Related antibodies
Assay kit Sample type Product code
CytoPainter ER Staining Kit - Green Fluorescence Adherent cells, suspension cells ab139481CytoPainter ER Staining Kit - Red Fluorescence Adherent cells, suspension cells ab139482
Related assay kits
Substance Description Product code
SP600125 JNK inhibitor ab120065TCS JNK 5a Selective JNK2/3 inhibitor ab120835Z-D(OMe)E(OMe)VD(OMe)-FMK Cell permeable caspase-3 inhibitor ab120488Z-VAD(OMe)-FMK Cell permeable, irreversible pan-caspase inhibitor ab120487Z-VAD-FMK Irreversible general caspase inhibitor ab120382
Related biochemicals
Protein Expression system Applications Product code
ATF6 protein (Tagged) Wheat germ ELISA, SDS-PAGE, WB ab131846Caspase 3 peptide Blocking ab38283
NFAT5 protein Wheat germ ELISA, SDS-PAGE, WB, other ab112396
PERK protein (Active) E. coli Functional Studies, SDS-PAGE, WB ab101115
Related proteins
Why choose Alexa Fluor® conjugated secondaries from Abcam?
• Tested extensively in our laboratories - guarantee bright staining and low background• Large range of pre-adsorbed antibodies - ensure low species cross-reactivity• Dilution range of 1/200 -1/1000 (10-2 µg/ml) in ICC/IF - perform at least 250 stainings• Antibodies conjugated to Alexa Fluor®488/550/594/647 - for your multi-color imaging experiments
Secondary antibodies
Key: AGMk - African Green Monkey, Dm - Fruit fly (Drosophila melanogaster), Hu - Human, Ms - Mouse, Ncra - Neurospora crassa, Rb - Rabbit, Rt - Rat
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
EX
T_002_13
Product
highlights
- Rabbit Monoclonal Antibodies (RabMAbs)