EGFR binding + D-K 6 L 9 lytic peptide

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EGFR binding + D-K 6 L 9 lytic peptide EGFR binding + Newly designed lytic peptide YHWYGYTPQNVIGGGLKL LKK LLK KLLK LL-NH 2 YHWYGYTPQNVIGGG KLL LKL LKK LLKL LKKK A B [θ] (deg cm 2 dmol -1 ) Wave length (nm) C D Fig. S1. (A)-(D) Kohno et al. PC SUVs PC/PS SUVs EGFR-D-K 6 L 9 peptide EGFR-lytic peptide [θ] (deg cm 2 dmol -1 ) Wave length (nm) PC SUVs PC/PS SUVs

description

A. B. EGFR binding + D-K 6 L 9 lytic peptide. EGFR binding + Newly designed lytic peptide. YHWYGYTPQNVIGGG KL L LK L L KK LLK L LKKK. YHWYGYTPQNVIGGGLK L LK K L LK KLL K LL-NH 2. C. D. EGFR-D-K 6 L 9 peptide. EGFR-lytic peptide. [θ] (deg cm 2 dmol -1 ). Wave length (nm). - PowerPoint PPT Presentation

Transcript of EGFR binding + D-K 6 L 9 lytic peptide

Page 1: EGFR binding + D-K 6 L 9  lytic peptide

EGFR binding + D-K6L9 lytic peptide EGFR binding + Newly designed lytic peptideYHWYGYTPQNVIGGGLKLLKKLLKKLLKLL-NH2 YHWYGYTPQNVIGGG KLLLKLLKKLLKLLKKK

A B[θ

] (d

eg c

m2 d

mol-

1)

Wave length (nm)

C D

Fig. S1. (A)-(D) Kohno et al.

PC SUVs

PC/PS SUVs

EGFR-D-K6L9 peptide EGFR-lytic peptide

[θ]

(deg c

m2 d

mol-

1)

Wave length (nm)

PC SUVs

PC/PS SUVs

Page 2: EGFR binding + D-K 6 L 9  lytic peptide

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Cel

l via

bilit

y (%

con

trol

)C

ell v

iabi

lity

(% c

ontr

ol)

H322MRC-5

Fig. S2. (A)-(B) Kohno et al.

Peptide (M)Peptide (M)

A

B

Peptide (M)Peptide (M)

BT-20 HC

● EGFR-D-K6L9○ D-K6L9

● EGFR-lytic○ D-K6L9

Page 3: EGFR binding + D-K 6 L 9  lytic peptide

A

27M-chimeric peptide

13M-chimeric peptide

24M-lytic peptide

12M-lytic peptide

Start injection

Stop injection

Time (s)

Res

pons

e

BH322

0.1mg/ml

0.05mg/ml

0.025mg/ml

MRC-5

CH322

Res

pons

e

0.1mg/ml

0.05mg/ml

0.025mg/ml

Res

pons

e

Time (s) Time (s)

Time (s)Time (s)

Res

pons

e

Res

pons

e

H322

MRC-5

Fig. S3. (A)-(C) Kohno et al.

0.1mg/ml

0.05mg/ml0.025mg/ml

Page 4: EGFR binding + D-K 6 L 9  lytic peptide

0.0

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1.0

MRC- 5 H322 BT- 20

rela

tive

KD

D

Fig. S3. (D) Kohno et al.

Page 5: EGFR binding + D-K 6 L 9  lytic peptide

Lytic-TAMRA

50m

50m

50m

0 min 2 min 5 min 10 min 20 min

Calcein-green

TAMRA-red

DIC

Fig. S4. Kohno et al.