Disease 2010 poster-final

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Diabetic Nephropathy Cancer / Angiogenesis Alzheimer’s Cardiovascular Disease COPD Neurohormonal Stress / Depression Membrane Damage Localized Cell Death JNK Pathway Activation Calpain Caspase3 p35 p25 CDK5 CT 26 CT 31 C-Terminal Fragments (CTFs) Tau Phosphorylation Neurofibrillary Tangles (NFTs) Microtubule Destabilization GSK-3β MAPK Pathway Activation Nitric Oxide ROS Microglial Activation + Inflammatory Response sAPPα sAPPβ α α Secretase APP β/γ Secretases γ β Amyloid Plaque Formation Amyeloid Precursor Protein (APP) Aβ 40 Aβ 42 Ca 2+ Fas-L Aβ c-Jun c-Fos Fas-L RAS RAF MEK ERK SHC GRB2 SOS PYK2 SRC PLC-γ STAT3 STAT1 DAG DAG FYN Growth Factor Receptors Angiotensin II Advanced Glycation End-Products SHP-2 JAK2 Electron Transport SHP-1 ROS STAT3 STAT1 ELK1 c-Fos c-Jun TGF-β PARP Fructose GAPDH PLD2 PKC NADPH NOX GP91 RAC p22 p67 p47 ROS NADP + TGF-β Glucose GLUT4 Glucose Autooxidation Aldose Reductase Sorbitol Polyol Pathway Sorbitol Dehydrogenase NADH NAD + DAG Pyruvic Acid Glycolysis Ca 2+ G aq AT1R Glomerularsclerosis Glomerular Mesangial Cell Growth Collagen IV RAGE RAS RAF MEK ERK VEGF Proteasome Ubiquitination p53 Degradation eNOS Nitric Oxide Ca 2+ IP 3 PLCγ Growth Signaling VEGF-R SHC GRB2 SOS PI3K AKT XIAP 14-3-3 BAD Bcl XL Apoptosis RSK VHL HuR Hypoxia Radiation ATM CK-1 MDM2 p53 MDM2 p53 Nuclear Import Thr18 Ser15 p53 mSin3A p53 p300 MDM2 VEGF mRNA Stabilization HIF-1α ARNT VEGF Radiation DNA Damage GROWTH ARREST GENES BAX p21 PARP-1 Inflammatory Gene Expression IL-6 TNFα Mucin Production IL-1β iNOS ROS Hypoxia-induced VEGF Production Natriuretic Peptides (NP) Particulate GC PDE Soluble GC PKG PKA Ca 2+ cGMP PDEs cAMP Multiple Targets NO iNOS HO-1 ROS CO Nitric Oxide Carbon Monoxide H 2 O 2 SOD O 2 - ONOO - Peroxynitrite Loss of Mitochondrial Membrane Potential Caspase Activation AIF EndoG CytC AIF EndoG DY L-Arginine COX-I/II AA PGG 2 PGH 2 TXA 2 Lipid Peroxidation TXA 2 Vasoconstriction DNA Fragmentation O 2 - Superoxide AKT PI3K ROS GSH GSSG MKK ASK1 p38 MAPK JNK Multiple Pathway Activation Glutathione Oxidation/Reduction GPX GR PGH 2 PGE 2 Synthase PGE 2 PGG 2 COX I/II AA PGE 2 Inflammation-Induced Lipid Peroxidation 8-iso-PGF 2α EGFR RAS RAF MEK ERK ROS PKC MSK-1 Keap-1 Nrf2 Nrf2 c-Fos c-Jun SOD HO-1 RAP-1 PKA cAMP EP 2/4 G as AC CREB NFκB RSK CREB CREB PKA ERK MEK cAMP β-catenin GSK-3 HRE Genes GR GR GR GR c c c c c c Enhanced Neuroplasticity Nuclear Import Bcl-2 BAD AKT RAF AKT DSHV AC B 2 AR Wnt PI3K IP 3 Hsp90 IM Glucocorticoid Receptor Activation RAS SOS Stress BDNF TrkB PIP2 DAG ACTH Glucocorticoids (e.g. Cortisol) Corticotropin Releasing Hormone HPA Axis Activation Norepinepherine G as Fz TrkB BDNF Bcl-2 Phosphodiesterase Inhibitor Antidepressants Phosphorylation BAD RAS RAF MEK ERK Disease-Associated Stress Signaling Proteins NAME NAME Transcription Factors Inhibition Gene Activation Activation Favored Many key signaling pathways determine whether cells are able to maintain homeostasis upon temporary or persistent exposure to stress. A variety of stressors including inflammation, an imbalance in nutrients, lack of oxygen (hypoxia), or improper folding of proteins, contribute to dysregualtion of critical pathways, ultimately resulting in disease. Enzo Life Sciences products are used by researchers around the world to investigate cellular stress signaling pathways involved in diabetes, cancer, cardiovascular disease, chronic obstructive pulmonary disease (COPD), Alzheimer’s disease and depression. ZZ-W0610-1001 8-iso prostaglandin F 2α Arachidonic acid Adenylyl cyclase Adrenocorticotropic hormone Apoptosis-inducing factor Amyloid precursor protein Aryl hydrocarbon receptor nuclear translocator Ataxia telangiectasia-mutated gene Amyloid beta Beta 2 adrenergic receptor Bcl2-antagonist of cell death B-cell lymphoma protein 2 Brain derived neurotrophic factor cyclic adenosine monophosphate Cyclin dependent kinase Cyclic guanosine monophosphate Cyclooxygenase Cyclic AMP response element binding C-terminal Cytochrome C Diacylglycerol Disheveled Epidermal growth factor receptor Endonuclease G Endothelial nitric oxide synthase Prostaglandin receptors Frizzled receptor Glyceraldehyde 3-phosphate dehyrogenase Guanylyl cyclase Glucose transporter 4 Glutathione peroxidase Glucocorticoid receptor (Neurohormonal Stress) Glutathione reductase (COPD) Reduced glutathione Gycogen synthase kinase Glutathione disulfide Hydrogen peroxide Hypoxia inducible factor Heme oxygenase-1 Hypothalamus pituitary adrenal Hormone response element Heat shock protein Hu antigen R Immunophilin Inducible nitric oxide synthase Inositol triphosphate Kelch-like ECH-associated protein 1 Mouse double minute 2 homolog Nuclear factor κ -light-chain-enhancer of activated B cells NADPH oxidase NF-E2-related factor 2 Phosphodiesterase Prostaglandin E 2 Prostaglandin G 2 Prostaglandin H 2 Phosphoinositide 3-kinase Phosphatidyl inositol bisphosphate Protein kinase A Protein kinase C Protein kinase G Phospholipase D 2 Reactive oxygen species Superoxide dismutase Signal transducer and activator of transcription Transforming growth factor beta Tyrosine receptor kinase B Thromboxane A 2 Vascular endothelial growth factor von Hippel-Lindau tumor suppressor X-linked inhibitor of apoptosis 8-iso-PGF 2α AA AC ACTH AIF APP ARNT ATM Aβ B 2 AR BAD Bcl-2 BDNF cAMP CDK cGMP COX CREB CT CytC DAG DSHV EGFR EndoG eNOS EP 2/4 Fz GAPDH GC GLUT4 GPX GR GR GSH GSK GSSG H 2 O 2 HIF-1 HO-1 HPA HRE Hsp HuR IM iNOS IP 3 Keap-1 MDM2 NFκB NOX Nrf2 PDE PGE 2 PGG 2 PGH 2 PI3K PIP 2 PKA PKC PKG PLD 2 ROS SOD STAT TGF-β TrkB TXA 2 VEGF VHL XIAP Abbreviations

Transcript of Disease 2010 poster-final

Diabetic NephropathyCancer / Angiogenesis

Alzheimer’s

Cardiovascular Disease

COPD NeurohormonalStress / Depression

MembraneDamage

LocalizedCell

Death

JNKPathway

Activation

CalpainCaspase3

p35

p25

CDK5

CT26 CT31

C-TerminalFragments

(CTFs)

Tau Phosphorylation

NeurofibrillaryTangles (NFTs)

MicrotubuleDestabilization

GSK-3βMAPK

PathwayActivation

NitricOxide

ROS

Microglial Activation +Inflammatory Response

sAPPα

sAPPβ

α

α Secretase

APP

β/γ Secretases

γ β

AmyloidPlaque

Formation

AmyeloidPrecursor

Protein(APP)

Aβ40

Aβ42

Ca2+

Fas-L

c-Jun c-Fos

Fas-L

RAS

RAF

MEK

ERK

SHC GRB2

SOS

PYK2

SRC

PLC-γ

STAT3 STAT1DAG

DAG

FYN

Growth FactorReceptors Angiotensin II

AdvancedGlycation

End-Products

SHP-2JAK2

ElectronTransport

SHP-1

ROS

STAT3 STAT1ELK1

c-Fosc-Jun

TGF-βPARP

Fructose

GAPDH

PLD2

PKC

NADPH

NOX

GP91RACp22

p67 p47

ROS

NADP+

TGF-β

Glucose

GLUT4

Glucose

Autooxidation

AldoseReductase

SorbitolPolyol

PathwaySorbitolDehydrogenase

NADHNAD+

DAGPyruvic

AcidGlycolysis

Ca2+

GaqAT1R

Glomerularsclerosis

GlomerularMesangial

Cell Growth

Collagen IV

RAGE

RAS

RAF

MEK

ERK

VEGF

Proteasome

Ubiquitination

p53Degradation

eNOS

NitricOxide

Ca2+

IP3

PLCγ

GrowthSignaling

VEGF-R

SHC GRB2

SOS

PI3K

AKT

XIAP

14-3-3

BAD

BclXLApoptosis

RSKVHL

HuR

HypoxiaRadiation

ATM

CK-1

MDM2

p53

MDM2

p53

NuclearImport

Thr18

Ser15

p53 mSin3A

p53 p300MDM2

VEGF mRNAStabilization

HIF-1α ARNT

VEGF

Radiation

DNA Damage

GROWTH ARREST GENESBAXp21

PARP-1

InflammatoryGene ExpressionIL-6TNFαMucin Production

IL-1βiNOS

ROS

Hypoxia-inducedVEGF Production

NatriureticPeptides

(NP)

ParticulateGC

PDE

SolubleGC PKG PKA

Ca2+

cGMP

PDEs

cAMP

MultipleTargets

NO

iNOS HO-1

ROS

CO

Nitric Oxide

CarbonMonoxide

H2O2

SOD

O2-

ONOO-

Peroxynitrite

Loss ofMitochondrial

MembranePotential

CaspaseActivation

AIF

EndoG

CytC

AIF

EndoG

DY

L-Arginine

COX-I/II

AA PGG2

PGH2

TXA2

LipidPeroxidation

TXA2

Vasoconstriction

DNAFragmentation

O2-

Superoxide

AKT

PI3K

ROS

GSH GSSG

MKKASK1

p38MAPK

JNK

MultiplePathway

Activation

GlutathioneOxidation/Reduction

GPX

GR

PGH2

PGE2Synthase

PGE2

PGG2

COX I/II

AA

PGE2

Inflammation-InducedLipid Peroxidation

8-iso-PGF2α EGFR

RAS

RAF

MEK

ERK

ROS

PKC

MSK-1

Keap-1

Nrf2

Nrf2 c-Fos

c-Jun

SODHO-1

RAP-1

PKA

cAMP

EP2/4

Gas AC

CREB NFκB

RSK

CREB

CREB

PKA ERK

MEK

cAMPβ-catenin

GSK-3

HRE Genes

GR

GR

GR

GR

c

c

c

c

c

c

EnhancedNeuroplasticity

NuclearImport

Bcl-2

BAD

AKTRAF

AKT

DSHV

AC

B2AR

Wnt

PI3K

IP3

Hsp90IM

GlucocorticoidReceptorActivation

RAS

SOS

Stress

BDNF

TrkB

PIP2

DAG

ACTH

Glucocorticoids(e.g. Cortisol)

CorticotropinReleasing Hormone

HPA AxisActivation

Norepinepherine

Gas

Fz

TrkBBDNF

Bcl-2

PhosphodiesteraseInhibitor

AntidepressantsPhosphorylation

BAD

RAS

RAF

MEK

ERK

Disease-Associated Stress Signaling

ProteinsNAME

NAME Transcription Factors

Inhibition

Gene Activation

Activation

Favored

Many key signaling pathways determine whether cells are able to maintain homeostasis upon temporary or persistent exposure to stress. A variety of stressors including infl ammation, an imbalance in nutrients, lack of oxygen (hypoxia), or improper folding of proteins, contribute to dysregualtion of critical pathways, ultimately resulting in disease. Enzo Life Sciences products are used by researchers around the world to investigate cellular stress signaling pathways involved in diabetes, cancer, cardiovascular disease, chronic obstructive pulmonary disease (COPD), Alzheimer’s disease and depression.

ZZ-W

0610

-100

1

8-iso prostaglandin F2α

Arachidonic acidAdenylyl cyclaseAdrenocorticotropic hormoneApoptosis-inducing factorAmyloid precursor proteinAryl hydrocarbon receptor nuclear translocatorAtaxia telangiectasia-mutated geneAmyloid betaBeta 2 adrenergic receptorBcl2-antagonist of cell deathB-cell lymphoma protein 2Brain derived neurotrophic factorcyclic adenosine monophosphateCyclin dependent kinaseCyclic guanosine monophosphateCyclooxygenaseCyclic AMP response element bindingC-terminalCytochrome CDiacylglycerolDisheveledEpidermal growth factor receptorEndonuclease G

Endothelial nitric oxide synthaseProstaglandin receptorsFrizzled receptorGlyceraldehyde 3-phosphate dehyrogenaseGuanylyl cyclaseGlucose transporter 4Glutathione peroxidaseGlucocorticoid receptor (Neurohormonal Stress)Glutathione reductase (COPD)Reduced glutathioneGycogen synthase kinaseGlutathione disulfi deHydrogen peroxideHypoxia inducible factorHeme oxygenase-1Hypothalamus pituitary adrenalHormone response elementHeat shock proteinHu antigen R ImmunophilinInducible nitric oxide synthaseInositol triphosphateKelch-like ECH-associated protein 1Mouse double minute 2 homolog

Nuclear factor κ-light-chain-enhancer of activated B cellsNADPH oxidaseNF-E2-related factor 2PhosphodiesteraseProstaglandin E2

Prostaglandin G2

Prostaglandin H2

Phosphoinositide 3-kinasePhosphatidyl inositol bisphosphateProtein kinase AProtein kinase CProtein kinase GPhospholipase D2

Reactive oxygen speciesSuperoxide dismutaseSignal transducer and activator of transcriptionTransforming growth factor betaTyrosine receptor kinase BThromboxane A2

Vascular endothelial growth factorvon Hippel-Lindau tumor suppressorX-linked inhibitor of apoptosis

8-iso-PGF2α

AAACACTHAIFAPPARNTATMAβB2ARBADBcl-2BDNFcAMP CDKcGMPCOX CREBCTCytCDAGDSHVEGFREndoG

eNOSEP2/4

FzGAPDHGCGLUT4GPXGRGRGSHGSKGSSGH2O2

HIF-1HO-1HPAHRE HspHuRIMiNOSIP3

Keap-1MDM2

NFκBNOXNrf2PDE PGE2

PGG2

PGH2

PI3KPIP2

PKAPKCPKGPLD2

ROSSODSTATTGF-βTrkBTXA2

VEGFVHLXIAP

Abbreviations