Cytarabine Analogues by Ion-Pairing LC-MS/MS · Cytarabine Analogues by Ion-Pairing LC-MS/MS...

ACE®

HPLC / UHPLC Columns

Cytarabine Analogues by Ion-Pairing LC-MS/MS

Reproduced with permission of Agilux Laboratories, USA

ACE 3 C18 3μm, 50 x 2.1 mm

Gradient analysis

A = 0.1% perfluoropentanoic acid +

0.1% formic acid in water

B = 0.1% perfluoropentanoic acid

+ 0.1% formic acid in acetonitrile

T (mins) %B

0 0

0.5 0

3.0 13

4.0 90

5.0 0

Flow rate: 0.7 ml/min

API 4000 MS

TurboIonSpray, positive mode

Source temperature: 550°C

Cytarabine and cytidine are

isobaric.

Robust method with good

separation achieved.

LLOQ = 1 ng/ml human plasma

© Advanced Chromatography Technologies Ltd. www.ace-hplc.com

1

ACE®


min 1 2 3 4 5 6 7

1 2

3

4 5

6

1a

Tricyclic Antidepressants

Key: 1 Doxepin

1a Doxepin isomer

1 Imipramine

2 Desipramine

3 Amitriptyline

4 Nortriptyline

5 Clomipramine

ACE Excel SuperC18

2μm, 100 x 3.0mm

Gradient analysis

A = 20 mM ammonium formate pH 3.0

B = 20 mM ammonium formate pH 3.0

in MeOH:water 9:1 v/v

Time (mins) %B

0 50

6 70

7 70

7.5

Flow rate: 1.2ml/min

Column temperature: 40°C

Injection volume: 2μl

Detection: UV, 260nm

2 © Advanced Chromatography Technologies Ltd. www.ace-hplc.com

ACE®


15-Hydroxy Lubiprostone in Human Plasma

ACE Excel 2 C18 2μm, 50 x 3.0mm

Isocratic analysis

A = 0.1% formic acid in water

B = Acetonitrile




MDS Sciex API 5000

TurboIonSpray negative mode

IonSpray voltage: -4500V

Source temperature: 450°C

15-Hydroxy lubiprostone

MW 392.5

Reproduced with permission of inVentiv Health Clinical, Quebec, Canada

Lubiprostone, a fatty acid derived from prostaglandin E1, is rapidly

metabolised to 15-hydroxy lubiprostone.

Quantitation is based on 15-hydroxy lubiprostone, with the d4 analogue

as internal standard

Transitions monitored:

15-Hydroxy lubiprostone

391.2 → 373.2

I.S. (15-Hydroxy lubiprostone-d4)

395.2 → 377.2

Lowest calibration standard sample containing 2.0pg/ml in human

EDTA K3 plasma

15-Hydroxy

lubiprostone I.S.


3

ACE®


Tricyclic Antidepressants

1

2

3 4

5

6

min 1 2 3 4 5 0

ACE Excel C18-PFP

2μm, 100 x 3.0mm

Isocratic analysis







Key: 1 Doxepin

2 Imipramine

3 Protriptyline

4 Nortriptyline

5 Trimipramine

6 Clomipramine


ACE®


1 2 3 4 5 6 7

ACE Excel C18-PFP

2μm, 100 x 3.0mm

Gradient analysis

A = 20 mM ammonium acetate pH 6.0

B = 20 mM ammonium acetate pH 6.0


Time (mins) %B

0 9

5.5 63

6.5 63

7 9





Paracetamol and Related Compounds

1

2 3

4

5

6

7

8

9

10

Key: 1 4-aminophenol

2 Hydroquinone

3 2-aminophenol

4 Paracetamol

5 2-acetamidophenol

6 Phenol

7 4-nitrophenol

8 2-nitrophenol

9 4-chloroacetanilide

10 4-chlorophenol


ACE®


Pravastatin and Isomers by LC-MS/MS

ACE C18 3 μm, 50 x 3.0 mm

Isocratic analysis

Acetonitrile-Methanol-THF-Water-

Acetic acid (15:20:5:60:0.1)

Flow rate: 0.6 ml/min

Column temperature: Ambient

Injection volume: 2 μl

Sample: 1 μg/ml each isomer

API 3000 triple quad MS

TurboIonSpray – negative mode

Extracted ion chromatogram of

MRM m/z 423.3 → 321.1

Reproduced with permission of Biotrial Bioanalytical Services, Laval, QC, Canada

All 3 compounds have MW 424

MS/MS conditions alone

insufficient for selective

quantitation

Baseline separation important

6-epi-Pravastatin

3α-iso-Pravastatin

Pravastatin


6

ACE®


NSAIDs

ACE 5 Super C18

5μm, 150 x 4.6 mm

Gradient analysis


B = 0.1% formic acid in acetonitrile

Time (mins) %B

0 20

20 70

25 70

26 20

Flow rate: 1ml/min




min 5 10 15 20 25

mAU

0

200

400

600

800

1000

Key: 1 Aspirin

2 Phenacetin

3 Sulindac

4 Tolmetin

5 Naproxen

6 Nimesulide

7 Flurbiprofen

8 Diclofenac

9 Phenylbutazone

10 meclofenamic acid

1

2

3

4

5

6

7

8

9

10


ACE®


Pristinamycin components in plasma by LC-MS/MS

Reproduced with permission of inVentiv Health Clinical, Quebec, Canada

ACE 3 C18 3μm, 30 x 3.0mm

Gradient analysis

A = 1mM NH4CO2H + 0.1%

HCO2H in 65:35 H2O:CH3CN

B = CH3CN T (mins) %B T (mins) %B

0 0 1.61 100

0.3 0 2.6 100

0.31 10 2.61 0

1.6 10 4 0

Flow rate: 1ml/min



MDS Sciex API 4000

TurboIonSpray positive mode

Low calibration standard containing

2.5ng/ml each of pristinamycin lA and llA

in human NaF/K2C2O4 plasma

Transitions monitored:

Pristinamycin lA 867.5 → 134.2

Pristinamycin llA 526.3 → 355.1

I.S. (Virginiamycin) 824.6 → 134.0

Processed study sample containing

pristinamycin lA and llA

Pristinamycin antibiotic is a mixture of 2 components –

pristinamycin lA and llA

Virginiamycin used as internal standard

Pristinamycin llA Pristinamycin lA


8

ACE®


NSAIDS – Fast analysis

ACE Excel Super C18

2μm, 50 x 3.0 mm

Gradient analysis


B = 0.1% formic acid in acetonitrile

Time (mins) %B

0 20

0.25 20

3.5 70

4 70

4.25 20



Injection volume: 1.4μl


Key: 1 Aspirin

2 Phenacetin

3 Sulindac

4 Tolmetin

5 Naproxen

6 Nimesulide

7 Flurbiprofen

8 Diclofenac

9 Phenylbutazone

10 meclofenamic acid min 0 1 2 3 4

mAU

0

100

200

300

400

500

600

700

1

2

3

4

5

6

7

8

9

10


ACE®


min 0 2 4 6

Diuretics

ACE Excel C18-PFP

2μm, 50 x 3.0 mm

Gradient analysis




Time (mins) %B

0 5

0.5 5

5 70

5.5 70

6 5





Key: 1 Hydrochlorothiazide

2 Amiloride

3 Furosemide

4 Indapamide

5 Bendroflumethazide

6 Spironolactone

1

2

3

4

5

6

Bendroflumethazide Spironolactone Indapamide Hydrochlorothiazide Amiloride Furosemide


ACE®


Diuretics (Isocratic)

ACE Excel C18-PFP

2μm, 50 x 3.0 mm

Isocratic analysis

10 mM ammonium formate pH 3.0

in methanol:water 45:55 v/v





Key: 1 Furosemide

2 Indapamide

3 Bendroflumethazide

4 Spironolactone

min 0 1 2 3 4 5

Furosemide Indapamide

Bendroflumethazide Spironolactone

1

2

3

4


ACE®


Antihistamines

1 2 3 4 5 6 7 8

Key: 1 Pseudoephedrine

2 Scopolamine

3 Doxylamine

4 Chlorpheniramine

5 Triprolidine

6 Diphenhydramine

7 Acrivastine

8 Promethazine

9 Fexofenadine

10 Cetirizine

11 Loratadine

ACE UltraCore SuperC18

2.5μm, 100 x 3.0mm

Gradient analysis




Time (mins) %B

0 0

7.5 100

8.5 100

9.0





1 2

3 4

5

6

7

8

9

11

10


ACE®


1 Analgesics / Cough & Cold Medicine Ingredients

mAU

min 4 5 6 7 8 9 10 11 12

1

2

3

4

5 6

7 8

9

10

11

12

13 14

15

1,2 3,4

5 6

7

8

9

10

11,12

13,14

15

ACE C18-AR

ACE C18

150x4.6mm, 3mm Gradient analysis A = 0.1% v/v formic acid (aq) B = 0.1% v/v formic acid in MeCN 40C 240nm 1.0mL/min T (min) %B 0 5 9 35 14 35 15 5

OH

NH CH3

O

1. paracetamol

OOH

NH2

2. 4-aminobenzoic acid

OOH

OH

N

NN

NO

CH3

O

CH3

CH3 NH CH3

OOH

OOH

OH

NH2O

OHNHCH3

O OH

OHO

O CH3

O

OOH

CH3 OH

O

OHO

OH

OCH3

NH CH3

O

HO

OH

3. 4-hydroxybenzoic acid

4. caffeine 5. 2-acetamidophenol

6. 3-hydroxybenzoic acid

7. salicylamide

8. acetanilide

9. phenol

10. aspirin

11. benzoic acid

12. sorbic acid

13. salicylic acid 14. phenacetin

15. salicylaldehyde


ACE®


Pharmaceutically Relevant Mixture

ACE UltraCore SuperC18: selectivity with pH:

14

DAD1 C, Sig=254,8 Ref=460,100 (F:\131024-MJ\131024_LOW_PH 2013-10-24 16-30-36\LOW0000005.D)

4

5

3

11

10

6

7

2 12

1

8,9

min 1 2 3 4 5 6

DAD1 C, Sig=254,8 Ref=460,100 (F:\131024-MJ\131024 2013-10-24 14-40-48\HIGH0000004.D)

4

5

3

11

6

7

2

12

1

8

9

10

min

50x2.1mm, 2.5mm Gradient analysis A1= 10mM HCOONH4, pH3 (aq) B1= 10mM HCOONH4, pH 3 in MeCN/water 9:1 v/v A2= 0.1% NH3, pH 10.7 (aq) B2= 0.1% NH3, pH10.7 in in MeCN/water 9:1 v/v T %B 0 3 5 100 6 100 40C 0.60 mL/min 254 nm

1. Atenolol 2. Methylphenylsulfoxide 3. Eserine 4. Prilocaine 5. Bupivacaine 6. Tetracaine 7. 1,2,3,4-Tetrahydro-1-naphthol 8. Carvedilol 9. Nitrobenzene 10. Methdilazine 11. Amitriptyline 12. Valerophenone

pH 3

pH 10.7


ACE®


Paraben Preservatives

Reproduced with permission of Trevor Gibson, The Chemical Analysis Facility, University of Reading

ACE 3 Phenyl, 150 x 2.1mm

Isocratic

Methanol – 25mM NH4OAc

(50:50), pH 6.8



Detection: UV at 240nm

Sample volume: 2μl

1. Methylparaben

2. Ethylparaben

3. n-Propylparaben

4. i-Butylparaben

5. N-Butylparaben

6. Benzylparaben

1

2

3

4 5

6


15

ACE®


Pharmaceutically Relevant mixture Separation

ACE UltraCore SuperPhenylHexyl: selectivity with pH:

16

7

6

4

7

10 2

8

1

11

9 5

3

11,8

3

4

7

10 2 1

9,6

5

pH 3

1 2 3 4 5 6 min

1. Benzamide 2. Caffeine 3. Procainamide 4. N-acetylprocainamide 5. Propiophenone 6. Toluene 7. Remacemide 8. Ethylbenzene 9. Carvdilol 10. Nortriptyline 11. Clomipramine

50x2.1mm, 2.5mm Gradient analysis A1= 10mM HCOONH4, pH3 (aq) B1= 10mM HCOONH4, pH 3 in MeOH/water 9:1 v/v A2= 0.1% NH3, pH 10.7 (aq) B2= 0.1% NH3, pH10.7 in in MeOH/water 9:1 v/v T %B 0 3 5 100 7 100 40 °C 0.60 mL/min 254 nm

pH 10.7


ACE®


Clonidine Hydrochloride

ACE UltraCore SuperC18

2.5μm, 50 x 4.6mm

Gradient analysis

A = 0.2% w/v phosphate buffer-CH3OH-

CH3CN (80:10:10)

B = CH3CN

Time (mins) %B

0 0

0.8 0

2.1 70

3.4 70

3.5 0

Flow rate: 2ml/min




Reproduced with permission of Mary Nguyen, Guys Hospital, London

1

3

2

1. Clonidine hydrochloride

2. Methyl hydroxybenzoate

3. Propyl hydroxybenzoate

Clonidine hydrochloride oral solution containing preservatives

Concentration clonidine HCl = 10μg/ml

Concentration Preservatives ~ 1.5g/ml


17

ACE®


Procaine and p-Aminobenzoic acid

Reproduced with permission of John Peace, Huddersfield Royal Infirmary, UK

ACE C18-PFP 3μm, 100 x 4.6mm

Isocratic analysis

Methanol - 0.6% acetic acid (19:81)

(adjusted to pH 4.7 with 20% NaOH)

Flow rate: 1ml/min


Procaine

p-Aminobenzoic acid

(potential degradant)

Detection limits for S/N = 10

Procaine = 0.0002mg/ml

p-Aminobenzoic acid = 0.00005mg/ml

Procaine – sterile manufactured product

System suitability tests


ACE®


150x4.6mm, 3mm Gradient analysis A = 20mM KH2PO4, pH 2.83 (aq) B = 20mM KH2PO4, pH 2.83 in 1:1 v/v MeOH:water 40C 205nm 1.5mL/min T (min) %B 0 20 15 70

min 0 2 4 6 8 10 12 14 16

mAU

0

25

50

75

100

125

150

175

Water Soluble Vitamins

200

1

2

3

4

5

6 7

N CH3

OH

NH2

OH

NH OH

CH3 CH3

HOH

O

OH

O

OOH

NH2

N

NHN

N

NH

NH

OOOH

O

OH

O

NH2

NH NH

S

O

H H O

OH

N

N

NH

N

CH3

CH3

O

O

OH

OH

OH

OH

1. Pyridoxine (vitamin B6) 2. Pantothenic acid (vitamin B5) 3. p-Aminobenzoic acid 4. Folic acid (vitamin B9 or vitamin M) 5. D-Biotin (vitamin B7 or vitamin H) 6. Cyanocobalamin (vitamin B12) 7. Riboflavin (vitamin B2)

Riboflavin (vitamin B2)

Pyridoxine (vitamin B6)

Pantothenic acid (vitamin B5)

p-Aminobenzoic acid

Folic acid (vitamin B9 or vitamin M)

D-Biotin (vitamin B7 or vitamin H)

Cyanocobalamin (vitamin B12)

ACE 3 C18-AR


ACE®


min 0 2 4 6 8

mAU

ACE 3 C18-AR

3um, 150 x 4.6 mm Isocratic analysis Flow rate: 1.0 mL/min Temperature: 22°C Detection: UV 260 nm

MP: 96.5:3.5 v/v 0.1% H3PO4 (aq) : MeOH

Water Soluble Vitamins / Polar Molecules

1

2

4

3

5

6

7

8

9

10

N CH3

OH

NH2

OH

Pyridoxamine or Vitamin B6

N

N N+

S

CH3CH3

NH2

OH

Thiamine or vitamin B1

N

NH2

ONicotinamide

OO

OHOH

OH

OHH

L-Ascorbic acid or vitamin C

OOH

NH2

4-aminobenzoic acid

N

ONH2

Isonicotinamide

NH

NH

O

OOH

O

Orotic acid

NH

N

N

NH

O

Hypoxanthine

N

O

OHOH

CH3

Pyridoxal or vitamin B6

N

OH

OH

CH3

OH

Pyridoxine or vitamin B6


ACE®


min 0 5 10 15 20 25 30

mAU

ACE 3 C18-AR

150x4.6mm, 3mm Gradient analysis A = 0.1% v/ formic acid (aq) B = 0.1% v/v formic acid in MeOH 40C 1.0mL/min 254nm T (min) %B 0 52 28 74 33 74

Non-Steroidal Anti-Inflammatory Drugs

1

2

4

3

5

6

7

8 9

*

NH

SNH

F

FF

S

O

NH2

OO O

Bendromethazide

OH

O

CH3O

Ketoprofen

OCH3

OH

O

CH3

Naproxen

CH3

OOH

CH3

SO CH3

Sulindac

CH3

CH3

CH3

OH

O

Ibuprofen

Cl

Cl

NH

OH

O

ClH

Diclofenac

N

OCH3

CH3

O

Cl

O

OH

Indomethacin

CH3

Cl

Cl

NH

OHO

Meclofenamic acid

CH3

CH3

NH

OHO

Mefenamic acid


ACE®


Complex Steroid Mixture Separation


ACE 3 C18-AR

ACE®


Chloroacetophenone halogenated isomers separation

23

ACE® C18-PFP™: Halogenated Positional Isomers


ACE®


1 Polar Analytes – Nucleosides & Vitamins

ACE C18-PFP, 3um, 150 x 4.6 mm

Isocratic analysis

Flow rate: 1 ml/min

Temperature: 22°C

Detection: UV 254 nm

Mobile phase: 20 mM H3PO4 (aq)

min 2 3 4 5

1

2

3

4

5

6

7 8

9

100% Aqueous

N CH3

OH

NH2

OH

Pyridoxamine or Vitamin B6

N

NH2

NH

O

Cytosine

N

N N+

S

CH3CH3

NH2

OH

Thiamine or vitamin B1

N

NH2

ONicotinamide

OO

OHOH

OH

OHH

L-Ascorbic acid or vitamin C

NH

NH

O

O

Uracil

N

N

NH2

OO

OHOH

OH

Cytidine

NH

N

N

NH

O

Hypoxanthine

N

N

NH2

OO

OH

OH

2-Deoxycytidine


ACE®


Separation of Sulphonamides

Reproduced with permission of Antonio Martin Esteban, National Research Institute of Agriculture and Food Technology INIA, Spain

ACE Excel C18-PFP

3μm, 150 x 4.6mm

Gradient analysis

A = Water

B = Acetonitrile

C = 10% formic acid

Time (mins) %A %B %C

0 84 15 1

12 74 25 1

14 59 40 1

16 84 15 1

18 84 15 1

Flow rate: 1ml/min


1

4

3

2

5 6

7

8

9

1. Sulfadiazine

2. Sulfapyridine

3. Sulfamerazine

4. Sulfamoxole

5. Sulfamethazine

6. Sulfamonomethoxine

7. Sulfachloropyridazine

8. Sulfamethoxazole

9. Sulfadimethoxine


ACE®


1. 2-Acetamidophenol 2. Acetanilide 3. Salicylamide 4. Aspirin 5. Phenacetin 6. Salicylic acid

ACE Excel CN-ES

ACE Excel CN

ACE CN-ES 50x2.1mm, 3mm Gradient analysis A = 0.1% formic acid B = 0.1% FA in MeOH 40 °C 0.6 mL/min 240 nm 5 – 38% B in 3.75 mins Hold at 38% B until 5 mins

Alternative selectivity with MeOH containing eluents

26

ACE Excel C18

Aspirin And Related Substances

min 0.5 1 1.5 2 2.5 3 3.5 4 4.5

1

2

3 4

6

5

min 0.5 1 1.5 2 2.5 3 3.5 4 4.5

1

2

3 4 6

5

min 0.5 1 1.5 2 2.5 3 3.5 4 4.5

1

3

2

4,6

5

OHO

O CH3

O

NH CH3

OOCH3

OHO

OH

NH2O

OH

NHCH3

O

OH

NHO

CH3


ACE®


1. Hydrochlorothiazide 2. Methylphenylsulphoxide 3. 1,3,5 Trinitrobenzene 4. Myricetin 5. P-Cresol 6. Sulindac 7. Toluene

Dipole + hydrophobic Interactions

Small Pharmaceutically Relevant Analytes

ACE Excel C18-PFP 1/2

2.096 3 2.401

5 3.529

4 3.898

7

4.678

6 4.736

ACE Excel C18-Amide 1/2 2.006

3 3.019

5 3.564

4

4.180

6

4.440 7 4.518

min 1 2 3 4

ACE Excel CN-ES 2 1.698

1 2.245

4 3.306

3 3.473 5

3.972

6

4.209 7 4.386

ACE CN-ES 100x3mm, 3mm Gradient analysis A = 20mM ammonium formate (aq) B = 20mM ammonium formate in MeOH 40C 0.6 mL/min T (min) %B 0 3 5 100 6 100 6.5 3

Polar embedded + hydrophobic Interactions

Hydrophobic + π-π + dipole + shape / position Interactions


ACE®


Salicylic acid in cell extracts

Extract from genetically engineered bacteria cells – targeted analysis for salicylic acid

Reproduced with permission of Trong Khoa Pham, University of Sheffield, UK

ACE UltraCore SuperC18, 5μm, 150 x 4.6mm

Gradient analysis (shown above)

A = 0.1% TFA in water

B = 0.1% TFA in acetonitrile

Flow rate: 1ml/min



28

ACE®


USP Guaifenesin

min 0.25 0.5 0.75 1 1.25 1.5 1.75

mAU

0

2

4

6

8

10

1

0.630

2

1.124

Key: 1 Guaifenesin

2 Benzoic acid

ACE UltraCore SuperC18, 2.5 μm, 50 x 3.0 mm

Isocratic analysis

H2O/MeOH/Glacial acetic acid (60:40:1.5 v/v)

Flow rate: 0.85 mL/min Column temperature: Ambient (22°C) Detection: UV at 276 nm

Injection volume: 1.5 μL


Translated method according to USP <621> guidance. Always check the latest and official method information from the relevant pharmacopoeia prior to analysis.

ACE®


USP Hydrocortisone

Key: 1 Hydrocortisone

2 Propylparaben

ACE Ultracore SuperC18, 2.5 μm, 100 x 4.6 mm

Isocratic analysis

MeCN/MeOH/H2O (25:25:50 v/v)

Flow rate: 1 mL/min

Column temperature: Ambient (22°C) Detection: UV at 253 nm


min 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5

mAU

0

20

40

60

80

100

120

1

2.537

2

4.601



ACE®


USP Estradiol

Key: 1 Ethylparaben

2 Estrone

3 Estradiol


Isocratic analysis

H2O/MeCN (45:55 v/v)

Flow rate: 1.39 mL/min



min 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5

mAU

0

50

100

150

200

1

1.096 2

1.511

3

1.804



ACE®


USP 17a-Ethinylestradiol

Key: 1 Ethylparaben

2 17α-Ethinylestradiol


Isocratic analysis

MeCN/H2O (50:50 v/v)




min 1 2 3 4

mAU

0

10

20

30

40

50

60

70

1

0.862

2

1.348



ACE®


USP Naproxen

min 1 2 3 4

mAU

0

5

10

15

20

25

1

1.054

2

1.855


Isocratic analysis

H2O with glacial acetic acid (49:1)/MeCN (50:50 v/v)




Key: 1 Naproxen

2 Butyrophenone



ACE®


USP Paracetamol / Aspirin / Caffeine

min 0 0.5 1 1.5 2 2.5

mAU

0

10

20

30

40

50 1

0.313

2

0.439 3

1.003

4

1.319

5

1.503

Key: 1 Paracetamol

2 Caffeine

3 Aspirin

4 Benzoic acid

5 Salicylic acid


Isocratic analysis

H2O with glacial acetic acid (69:3)/MeOH (72:28 v/v)

Flow rate: 2 mL/min


Detection: UV at 275 nm




ACE®


USP Hydroquinone

min 0.25 0.5 0.75 1 1.25 1.5 1.75

mAU

0

20

40

60

80

0.606

Key: 1 Hydroquinone

ACE Excel C18, 2 μm, 50 x 3.0 mm

Isocratic analysis

H2O/MeOH (45:55 v/v)





35

OH

OH


ACE®


USP Doxepin

min 5 10 15 20 25

mAU

0

2.5

5

7.5

10

12.5

15

17.5

20.193

22.715

Key: 1 Doxepin isomer

2 Doxepin isomer

ACE C8, 3 μm, 50 x 4.6 mm

Isocratic analysis

0.2 M Monobasic NaH2PO4 in water pH 2.5:MeOH

(30:70 v/v)






36

O

N

CH3

CH3


ACE®


USP Metronidazole

min 0.5 1 1.5 2 2.5 3 3.5

mAU

0

100

200

300

400

500

600

700

2.301

Key: 1 Metronidazole

ACE C8, 3 μm, 75 x 4.6 mm

Isocratic analysis

H2O/MeOH (4:1 v/v)

Flow rate 1.0 mL/min



Injection volume: 15 μL


37

N

N

CH3O2N

OH


Cytarabine Analogues by Ion-Pairing LC-MS/MS · Cytarabine Analogues by Ion-Pairing LC-MS/MS...

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