CONFIDENTIAL AND PROPRIETARY...CONFIDENTIAL AND PROPRIETARY Ÿnmealamino acids & fatty acids...

CONFIDENTIAL AND PROPRIETARY1

CONFIDENTIAL AND PROPRIETARY

LABTESTING PROTOTYPE PILOTPLANTS DEMOPLANTINDUSTRIAL

PLANT

Funded

OutsourcedPrototype

First TestLab

Pilot Plant1.0No automation

Pilot Plant2.0Semiautomation

Ϋnsite DemoPlantFull automation

Ϋnfarm#1IndustrialPlant

BEST IN CLASS R&DPARTNERSHIPS

2011 2012 2013 2014 2016 20202015

COFOUNDER

Demonstrated scalability over time

$200m IN FUNDING, GRANTS and DEBT

+$25MEUGRANT-2019


#scalability#robotics#bigdata#verticalindoorfarming

25 patents(Process, Products,

Applications)


It’s not only about proteins !

Automated Farming

(“Upstream”)

Automated Processing

(“Downstream”)

Crops By-Products

WheatCorn

BarleyPea

PotatoCassava

Rice

INPUTS OUTPUTS

Proteins

ŸnMealTM

OIL

ŸnOilTM

FERTILIZER

ŸnFrassTM

Premium ingredients

Ÿnfarm(Ÿnsect plant)

1. Reproduction2. Growth3. Feeding & Sorting

4. Sterilisation5. Pressing & Drying6. Packaging

END MARKETS

Plant fertilizer

Aquafeed

Pet food


Fat 13%

Pepsin digestibility >86%

Crude protein 70%

Ash 3%

ŸnMeal : Defatted free flowable Protein


75% protein < 12.4 kDa

kDa

mAU

5 10 15


Ÿnmeal amino acids & fatty acids composition

Amino acids Unit ŸnMeal Fatty acids Unit ŸnMeal

Arginine % 2.56 C12:0 % 0.03

Histidine % 1.39 C14:0 % 0.22

Isoleucine % 2.11 C15:0 % 0.01

Leucine % 3.99 C16:0 % 1.33

Lysine % 3.32 C16:1 % 0.05

Threonine % 1.87 C16:1n-7 % 0.16

Tryptophan % 0,6 C17:0 % 0.02

Valine % 2.91 C17:1 % 0.01

Methionine % 0,84 C18:0 % 0.35

Cysteine % 0.63 C18:1n-9 % 3.03

Phenylalanine % 1.98 C18:1n-7 % 0.04

Tyrosine % 2.68 C18:2n-6 % 2.96

Taurine % 0.42 C18:2tn-6 % 0.02

Aspartic acid + Asparagine % 4.51 C18:3n-3 % 0.14

Glutamic acid + Glutamine % 6.36 C20:0 % 0.02

Alanine % 3.83 C20:1n-9 % 0.01

Glycine % 2.54 C20:2n-6 % 0.01

Proline % 3.18 C20:3n-6 %

Serine % 2.94 C20:4n-6 %

C20:3n-3 %

C20:4n-3 %

C20:5n-3 %

C22:0 % 0.01

C22:2n-6 %

C22:4n-6 %

C22:5n-6 %

C22:5n-3 %

C22:6n-3 %

11


Ÿnmeal is a safe and premium product

8

✓ High total nucleotides content: 2,9g/kg

Growth

✓ Health properties

✓ Palatability (1.5g/kg of Adenosine: active attractive response)

✓ Contaminant check: free of contaminant

✓ Very low total volatile nitrogen (TVN) => high level of freshness

✓ Very low heavy metal content

✓ Free from pesticide

Feeding trials


Immunology

Growth trial

Shrimp trial : Material & Methods

16

• Juveniles L. vannamei (1.6 g initial weight)

• Control diet with 25% Fish meal inclusion

• 5 diets (up to 100% FM replacement)

• Feeding during 8 weeks (Thailand)

Fed for 8 weeks (Fish meal inclusion of 25%)

-Thirty shrimps from each treatment

-Challenge with pathogenic bacteria, Vibrio parahemolyticus,

4.3X105*CFU/ml (4.6logCFU/ml) intramuscular injection

-Mortality was recorded for 10 days

-The clearance ability was determined.


Shrimp diet formulation

11

Material in Formula T1 T2 T3 T4 T5Fishmeal 30.0 15.0 10.0 5.0 0.0Soybean 15.0 15.0 15.0 15.0 15.0Wheat gluten 8.0 8.0 8.0 8.0 8.0Squid liver powder 8.0 8.0 8.0 8.0 8.0Wheat flour 24.0 24.0 24.0 24.0 24.0Broken rice 2.2 1.2 1.2 1.2 1.2Soy protein concentrate 0.0 15.0 15.0 15.0 15.0ŸnMeal 0.0 0.0 5.0 10.0 15.0Corn gluten 5.0 5.0 5.0 5.0 5.0Tuna fish oil 0.5 0.5 0.5 0.5 0.5Soya oil 2.0 2.0 2.0 2.0 2.0Soy lecithin 2.0 2.0 2.0 2.0 2.0Mono-cal 0.0 1.0 1.0 1.0 1.0Binder 1.7 1.7 1.7 1.7 1.7Vitamin premix 1.6 1.6 1.6 1.6 1.6Sum 100.0 100.0 100.0 100.0 100.0

Chemical composition by (AOAC,2000)Moisture (%) 7.38 7.14 7.09 7.25 7.18

Protein (%) 37.38 36.88 37.06 37.84 38.81

Lipid (%) 7.18 6.50 6.97 7.23 7.60

Fiber (%) 2.69 2.95 3.09 3.33 3.68

Ash (%) 10.39 8.02 7.70 6.58 5.96

Energy (Kcal/Kg)4560.14 4467.36 4477.59

4487.82

4498.05


ŸnMeal improves performances

12

0.00

1.00

2.00

3.00

4.00

5.00

6.00

2wks 4wks 6wks 8wks

Weight gain

T1 (control) T2 (25%) T3 (50%) T4 (75%) T5 (100%)

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

T1(ctrl)

T2(25%)

T3(50%)

T4(75%)

T5(100%)

FC

R

FCR

A B B B B

+33,7%

-25%+21%

Addition of ŸnMeal lowered the feed

conversion ratio by 25% at day 60

A B B B B


Challenge test vs Vibrio : ŸnMeal increases survival rate

13

4.33a

4.25ab

4.03bc

3.82cd

3.72d

3.4

3.5

3.6

3.7

3.8

3.9

4

4.1

4.2

4.3

4.4

0

20

40

60

80

100

120

T1 (control) T2 (25%) T3 (50%) T4 (75%) T5 (100%)

Cle

ara

nc

e a

bili

ty

(lo

g C

FU

/ml)

Ph

en

ol o

xyd

ase

ac

tivity

(un

it/m

g p

rote

in)

Phenol oxydase activity vs clearance ability of the

bacterial pathogen

Phenol oxydase acitivity clearance ability

56.67

70

90

83.3386.67

0

10

20

30

40

50

60

70

80

90

100

T1 T2 (25%) T3 (50%) T4 (75%) T5 (100%)

Survival rate (%)

a ab bc cd d

c cb a ab ab

Survival rate is increased by up to 60 % vs control diet


Products from Tenebrio Molitor show superior results

Trials

Mice

Poultry

Rainbow Trout

Atlantic Salmon

Protocol

Full cycle 45 days

Juveniles3-month test

Juveniles3-month test (pre

& post smolt)

Substitution

Caseine meal:

0 to 100 %

Soy meal: 0 to 100 %

Fishmeal: 0 to 100 %

Fishmeal: 0 to 100 %

Equal

+2%

+34%

+5%

Weight gain

Not measured

Not measured

-18%

Not measured

FCR

Cholesterol content in liver

drastically reduced

-15% water consumption

(better leg skin)

Not measured

-7% mortality

Health

Not measured

+2%

Not measured

+5%

Filet yield

ŸnMeal Test Results ŸnFrass Test Results

Trials

Rapeseed

Wheat

Corn

Protocol

Full cycle from seed to harvest



Substitution

Chemicals : 50 to 100 %



+300%

+230%

+140%

Dry biomass/ha

Not measured

+70%

Not measured

Tillers number


CONFIDENTIAL AND PROPRIETARY...CONFIDENTIAL AND PROPRIETARY Ÿnmealamino acids & fatty acids...

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