Circulating levels of the adipokines monocyte chemotactic protein-

4(MCP-4), Macrophage inflammatory protein-1β (MIP-1β) and Eotaxin-3

in sever obesity and following bariatric surgery.

Presented by : Sumaiah Ali Al-Ghamdi

:Submitted toDr.Suliman Al-Omar

A. Gentili, M. S. Zaibi, S. Y. Alomar, S. De Vuono, M. A. Ricci, A. Alaeddin, D. Siepi, M. Boni, G. Vaudo, P. Trayhurn, G. Lupattelli

publishing in Thieme journal in 2016

Content

• Introduction • Goals of work • Subjects & method • Result • Dissections

Goals of work

Investigate the involvement of the adipokines eotaxin-3,(MIP-1β)macrophage inflammatory protein-1β , and( MCP-4) monocyte chemotactic protein-4 in obesity and related comorbidities and the modification of their circulating levels after bariatric surgery.

Subjects & Method

Subjects:• 80 obese patients(25 male -55 female in age

between 11-43)in list of bariatric surgery (laparoscopic sleeve gastrectomy -LSG) were examined at the beginning of the research and 10 months after LSG, while normal-weight controls were examined only once at beginning of the research.

• 20 control (9 male -11 female ,age between 12-48)

Subjects & MethodMethod: Anthropometric, clinical and laboratory

parameters Measurements of plasma levels of eotaxin-3,

MIP-1β, MCP-4, leptin and adiponectin :• Blood was collected then plasma was separated

using centrifuge .• Leptin and adiponectin were measured by an

enzyme-immunoassorbent assay (ELISA) .• Eotaxin-3, MIP-1β, MCP-4 were measured by

(ECL) electrochemiluminescence immunoassay.

Subjects & Method

• Electrochemiluminescence permits the detection and measurement of a biological or chemical substance within a sample.

Subjects & Method

Ruthenium and TPA are oxidized at the surface of the electrode when voltage is applied. The TPA loses a proton, which reduces the ruthenium to an excited state, causing light to be emitted. Multiple excitation/emission cycles amplifies the light emitted and increase sensitivity. The emitted light is measured to determine concentration of analytes in sample.

Subjects & Method Instrumental analysis: Intima-media

thickness measurementCarotid arteries ( right and left ) were examined by

high-resolution B-mode ultrasonography using ultrasound device.

Statistical analysis

Result

A. all the anthropometric , clinical and metabolic characteristics are higher in the obese group before-surgery in comparative with the control except (TC, LDL-C, HDL.C-TG and ApoB) .

after surgery , all the anthropometric , clinical and metabolic characteristics are highly decreased except HDL.C increased and TC, LDL-C has no changes.

ResultB. Circulating levels of chemokines, leptin and

adiponectin

Obese group– Post surgery

Obese group before surgery

control

decreased higher normal MCP-4

decreased No differences No differences

Eotaxin-3

decreased No differences No differences

MIP-1β

increased decreased normal Adoponectin

decreased increased normal leptin

ResultC. Correlation between circulating levels of

chemokines and other parameters in the obese group

Obese group pre surgery

+ BMI MCP-4

+LDL-C, apoB, Leptin Eotaxin-3

+age , right and left IMT, Leptin

- Adiponectin

MIP-1β

Disscation • MCP-4 considered as biological evidence for the

obesity because their decreasing with the decrease of BMI

• it is play indirect role in obesity related diseases by affecting the other chemokines (eotaxin -3 and MIP- 1β)

• MIP-1β and eotaxin-3 are Play an important role in diseases associated with obesity, such as inflammation and atherosclerosis and heart and blood vessels disorders .

Thank you