Chapter 3 Alpha-domain structures -...

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Chapter 3 Alpha-domain structures Coiled-coil Helical bundle Globin fold

Transcript of Chapter 3 Alpha-domain structures -...

Page 1: Chapter 3 Alpha-domain structures - KOCWcontents.kocw.net/KOCW/test/document/2013/skku/Leesangho/3.pdf · structures and are sometimes important for the formation of heterodimeric

Chapter 3 Alpha-domain structures

Coiled-coil Helical bundle

Globin fold

Page 2: Chapter 3 Alpha-domain structures - KOCWcontents.kocw.net/KOCW/test/document/2013/skku/Leesangho/3.pdf · structures and are sometimes important for the formation of heterodimeric

COILED-COIL STRUCTURES

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Examples: mammals' hair (including wool), hooves, nails, rhinoceros horns, much of the outer layer of skin

α-Keratin: a Coiled Coil

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Coiled-coil α helices contain a repetitive heptad amino acid sequence pattern

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Salt bridges can stabilize coiled-coil structure

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Packing side chains in the hydrophobic core of coiled-coil structure: “knobs in

holes” model

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Coiled Coils in The Nature: Protein Binding

[Marsden and Kros (2010) Angew. Chem. Int. Ed.]

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Coiled Coils in The Nature: Structural Functions

[Marsden and Kros (2010) Angew. Chem. Int. Ed.]

NuMa (Nuclear Mitotic Apparatus): a fibrous nucleoskeletal protein

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Coiled Coils in The Nature: Dynamic Functions

[Marsden and Kros (2010) Angew. Chem. Int. Ed.]

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HELIX BUNDLE STRUCTURES

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The four-helix bundle is a common domain structure in α proteins

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Alpha-helical domains are sometimes large and complex

Bulgecin, a glycopeptide inhibitor

70-kDa soluble lytic transglycosylase (SLT70), a target for bulgecin

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GLOBIN FOLD

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The globin fold is present in myoglobin and hemoglobin

The globin fold has been preserved during evolution

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Ridges of one α helix fit into grooves of an adjacent helix

ridge

groove

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The “ridges in grooves” model

Globin fold

Four-helix bundle

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Sickle-cell hemoglobin confers resistance to malaria: E6V mutation

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