Chapter 19

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Oxidative Phosphorylation Part 2 Chapter 19

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Chapter 19. Oxidative Phosphorylation Part 2. Oxidative Phosphorylation Part 2. Key Topics: To Know How cells deal with reactive oxygen species (ROS). Calculating Δ G o ’ of the Proton Motive Force. Membrane ATPase and how it works. Cytoplasmic NADH getting into the mitochondria. - PowerPoint PPT Presentation

Transcript of Chapter 19

Page 1: Chapter 19

Oxidative Phosphorylation

Part 2

Chapter 19

Page 2: Chapter 19

Oxidative Phosphorylation Part 2

Key Topics: To Know

1.How cells deal with reactive oxygen species (ROS).2.Calculating ΔGo’ of the Proton Motive Force.3.Membrane ATPase and how it works.4.Cytoplasmic NADH getting into the mitochondria.5. Adenylate Control.6. Mitochondia and apoptosis.7.PMF can be used to ?

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ROS : Reactive Oxygen Species

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ΔG of the Proton Motive Force

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Nobel Prize for Chemiosmotic Model = PMF

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Converting PMF to ATP

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Mitochondrial Respiration+Phosphorylation

EOC Problem 9: Compartmentalization of Citric Acid Cycle.

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Adjusting the pH Generates ATP

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Molecules that Collapse the PMF

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DNP is an Uncoupler

EOC Problem 6: Figuring out what happens with uncouplers.

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Odorous Flowers that Heat Up Using Uncoupled Respiration

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Worked out F1 ATPase kinetic mechanism + First purified the FoF1 ATPase

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ATP Synthase

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ATP Synthase Catalyzes by Steps

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F1 Fo ATP synthase

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F1 Complex

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Used to Inhibit ATP Synthase Reaction

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F1 ATPase Spinning

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ATP Synthase Seen Rotating !

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ATP Synthase Substrate Transport

ADP and Pi are products of Anabolism

EOC Problem 11 is the

effect of ADP and Pi on ATP

Synthesis.

Get Ready, NADH formed in Cytoplasm

in Next

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Glycolysis NADH Enters the Mitochondria by the Malate Aspartate Shuttle

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NADH from Glycolysis Can Also Get in by Glycerol Phosphate DH

Also has a Anabolic Role

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EOC Problems 13 and 14: Get in to the Pasteur Effect and “Petites” in Yeast Colonies. Fun stuff.

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Adenylate Control of Glycolysis/CAC/e-transport

1. Acceptor Control: availability of ADP + Pi

2. Mass action ratio: [ATP]/([ADP] [Pi])

EOC Problem 17 Gets into the rate of ATP turnover in heart muscle…the muscle that always has to be “on”.

EOC Problem 18 Gets into the same in insect flight muscle.

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Inhibitory Protein IF1 Stops Loss of ATP During Ischemia that is Hypoxia

IF (red and white) forms dimers at pH 6.5 to Stop Rotation of ATP Synthase

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Thermogenin – Protein Uncoupler in Brown Fat

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Mitochondria in Adrenal Gland: P-450 Oxygenases Specialized for Steroid Synthesis

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Electron Flow to P-450

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Apoptosis is Regulated by the

Mitochondria

Initiates a series of proteasess

Stress, Damage

EOC Problem 19: relates mitochondrial function to cancer. Hint think about the first couple of slides.

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Escherichia coli Electron Transport

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Bacterial Quinone

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Bacteria Use the PMF to Rotate Their Flagella

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Proton Motive Force Functions To

1. Synthesize ATP (from ADP + Pi).

2. Active Transport (Symports, Antiports, Uniports; review Chapter 11).

3. Rotate Bacterial Flagella.

4. Reversed Electron Transport (some Photosynthetic and Chemoautotrophic Bacteria)

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Things to Know and Do Before Class1. How cells deal with reactive oxygen

species (ROS).

2. Calculating ΔGo’ of the Proton Motive Force.

3. Membrane ATPase and how it works.

4. Cytoplasmic NADH getting into the mitochondria.

5. Adenylate Control.

6. Mitochondria and apoptosis.

7. PMF can be used to ?

8. EOC Problems: 6, 9, 11,13,14, 17, 18, 19.