ChampionTM Competent Celldocs.smobio.com/document/doc/Supplement/CC_180817.pdf · 2018. 9. 21. ·...
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Champion TM Competent Cell E-mail: [email protected] | www.smobio.com ● High Transformaon Efficiency > 1 x 10 - 10 cfu/μg pUC19 DNA Ideal for all cloning and library preparaon ● Time Saving, Easy-to-Use Transformaon needs only 20 seconds to 6 minutes. Heat shock and recovery procedures oponal. ● No Expensive Equipment needed No need for an expensive electroporator and disposable cuvees Mix then Spread - Saving Time 9 8 NEW! NEW!
Transcript of ChampionTM Competent Celldocs.smobio.com/document/doc/Supplement/CC_180817.pdf · 2018. 9. 21. ·...
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ChampionTM
Competent Cell
E-mail: [email protected] | www.smobio.com
● High Transformation Efficiency > 1 x 10 - 10 cfu/μg pUC19 DNA
Ideal for all cloning and library preparation
● Time Saving, Easy-to-Use Transformation needs only 20 seconds to 6 minutes.
Heat shock and recovery procedures optional.
● No Expensive Equipment needed No need for an expensive electroporator and disposable cuvettes
Mix then Spread - Saving Time
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NEW!NEW!
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E-mail: [email protected] | www.smobio.com E-mail: [email protected] | www.smobio.com
Quickly thaw
10 secs
Homemade Competent Cells
Champion™ Competent Cells
10 secsQuickly thaw
1 sec
1 sec 45 secs 5 mins
Incubate on ice
Quickly thaw Mix 1 sec
Spread
AddPlasmid
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Notice: the antibiotic concentrationfor Champion™ Competent Cells
Ampicillin (Ap)Kanamycin (Km)Tetracycline (Tc)Chloramphenicol (Cm)
20 μg / ml25 μg / ml7.5 μg / ml20 μg / ml
Quickly thaw the ChampionTM Competent Cells with hand (body temperature), warm water bath or running tap water about 10~20 seconds until 1/3~1/2 volume is thawed.
Add less than 10% of competent cell volume DNA (ex. ligation products) to cells. Vortex 1 sec or tap the tube with finger to mix well. Incubate on ice for 0~10 min. (The ice-incubation will slightly increase efficiency) Plate the bacteria/DNA mixture onto a pre-warmed (room temperature to 37°C) and dried selective LB agar plate (LB + antibiotic). Incubate the plates at 37°C until the colonies are suitable for analysis.
Time-saving Transformation Protocol
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*standard transformation protocol can be found in product information
Incubateon ice
Heatshock
Incubateon ice
Cellrecovery
Spread30 mins - 1 hr
Addplasmidand mix
0-10 minsIncubateon ice
Spread
5-30 mins
Items and ordering information
Product Name Compatible to E. coli strain Efficiency (cfu/μg) Quantity Cat. No.
Champion™ 109 High E. coli JM109 >1 x 108 100 μl x 80 vials CC0202
100 μl x 24 vials CC0204
Champion™ 21 E. coli BL21 (DE3) >1 x 107 100 μl x 80 vials CC2102
100 μl x 24 vials CC2104
Champion™ DH5α High E. coli DH5α >3 x 108 100 μl x 80 vials CC5202
100 μl x 24 vials CC5204
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