Advances in CNS and Brain Research

The adhesion molecule Cadherin-11 anchors β-catenin and drives fibroblast cellfunction in rheumatoid arthritis. This work aimed to clarify the involvement ofCDH11 and β-catenin signaling during synovial fibroblast proliferation, usinginduced cell proliferation, siRNAs, neutralizing antibody and ELISA. Yoshiokaand colleagues report that CDH11-mediated β-catenin signaling was involved inIL-1β-induced cell proliferation, but not in proliferation induced by TNF-α. Thework underscores the utility of human fibroblast-like Synoviocytes for the studyof molecular mechanisms behind rheumatoid arthritis.

Quantitative analysis of cadherin-11 and β-catenin signalling duringproliferation of rheumatoid arthritis-derived synovial fibroblast cells

Human Fibroblast-LikeSynoviocytes from rheumatoidarthritis patients were used in thisstudy

The blood–brain barrier inhibits drugs and genetic material fromreaching the brain, challenging the development of efficientcarriers. This study targets DNA to brain tissue using a novelgene delivery system. A peptide was linked on dendrimers, andDNA was loaded in functionalized nanoparticles, followed bybiodistribution analysis. The nanoparticles exhibit goodtransfection efficacy with low toxicity, and CAI-supplied braincapillary endothelial cells internalize the nanoparticles byendocytosis. The results suggest such nanoparticles might providesafe, effective gene delivery to the brain.

Journal of Pharmacy and Pharmacology. doi: 10.1111/jphp.12410

4. Smooth Muscle Cells and Atherosclerosis

1. Growth Factors and Carcinoma Pathology

HFLS-RA:

Hot Tech: Disease Models

4

Highlight Study

Stanford researchers have generated tiny, spherical, free-floating balls of functional human neuronal braincells that mimic the architecture of the cerebral cortex. Hundreds of the 3-D “human cortical spheroids”can be easily made using a single human skin sample. They spheroids exhibit neural network activity,contain astrocytes and working synapses, and express genes in patterns similar to a developing humanbrain. The work makes functional human brain tissue available to study brain development and molecularcauses of neuropsychiatric diseases such as autism and schizophrenia.

Pasca et al., Nature Methods

Atherosclerosis involves SMC invasion & proliferation in plaques. Integrins andsoluble factors drive SMC proliferation, differentiation, migration and invasion.

3

3. Cancer drugs that wake up sleeping killer T cellsDrugs that disable checkpoint proteins are showing a keen abilityto awaken T cells and, in so doing, pull away cancer’s veil

e-Newsletter

2. Radioprotective Properties for Endothelial Cells

Issue 8

Bovine Aortic Endothelial Cells from cellapplications.com demonstrateradioprotective properties of Phosphine-borane complex 1

Study using Anti-IGFBP3 Ab examines Involvement of insulin-like growthfactor-binding protein 1 complexes in pathology of colorectal carcinoma

Aug 2015

Time-lapse video of HFLS-RA

Zarebkohan et al., Eu J Pharm Sci

Nature MethodsJ Neuroscience

Neuroscience News Cell Biol Res

1 2

1. Regeneration inPrimary Human

MammaryEpithelial Cells

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Can iPSC be generated from fibroblasts?

Organoid regeneration assay: Humanmammary epithelial cells cultured in

collagen gel matrix environment.Branched structures resemble

functional units of mammary gland.

CAI Support, Downloads

Cool ImagesTrending: Tissue Engineering

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Oct 31, 2015

Yes, Human Dermal Fibroblasts(HDF) from Cell Applications,Inc can be used to establishinduced pluripotent stem cells(iPSC). For instance, seemethod in recent patentapplication 20150175973 A1 byNobel winner S. Yamanaka, andO. Keisuke:

1. HelmholtzZentrum München

and Literature

FAQ

Primary Cells in Training Course Primary Cells on Sci Photo Library

Khan et al, J Immunol

2. Vanderbilt U 3. Michigan Tech

2. Tunable shapememory actuator

for vascularapplications

Tunable thermo-responsive shapememory polymers for biocompatible,minimally-invasive medical devices.

Study uses human endothelial cells &media from CAI.

3. ThreeDimensional

Bioprinting ofNerve Cells

Hopes for 3D bioprinter to synthesizenerve tissue, regenerate damaged

nerves for spinal cord injuries. Idea isto let nerve pass through small holes

in scaffold, similar to vertebrae.

Mitochondria Function

W Li, Natl Inst Health

Highly Efficient Methodfor Establishing Induced

Pluripotent Stem Cell

www.cellapplications.com

News from the Cell Industry

In 1994, Nobel laureate S. Hell & coworkersintroduced stimulated emission depletionnanoscopy, offering unprecedentedpossibilities to investigate sub-cellularstructure and function. STEM breaks thediffraction barrier by “squeezing” the effectivefluorescence volume of a scanningmicroscope via a process called stimulatedemission. This article overviews the nextgeneration of STED nanoscopes and cutting-edge implementations, with insight into thefuture, where everything is imaged faster, withunprecedented sensitivity, and label-free.

The CAI Book & Journal Club

“A revolution influorescencemicroscopy”

STED nanoscopy:a glimpse into the futureGet Recognized for your Cell Images!

“Seeing isbelieving”

NSF Visualization Challenge - Voting Nov

Wellcome Image Awards - Deadline Sep 9

Primary Cells Media & Reagents Cell Biology Products