AVEQ final Meeting – Bucarest, 18-22 October 2010
14
AVEQ final Meeting – Bucarest, 18-22 October 2010 WP7: β-GLUCAN AND FIBRE ANALYSIS Daniela Sgrulletta Sivia Bellato Viviana Del Frate Rita Redaelli
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WP7: β -GLUCAN AND FIBRE ANALYSIS Daniela Sgrulletta Sivia Bellato Viviana Del Frate Rita Redaelli. AVEQ final Meeting – Bucarest, 18-22 October 2010. MATERIALS & ANALYSES. 3 locations each year All the genotypes: total β-glucan (P2) 70 selected genotypes: soluble β-glucan (P6) - PowerPoint PPT Presentation
Transcript of AVEQ final Meeting – Bucarest, 18-22 October 2010
AVEQ final Meeting – Bucarest, 18-22 October 2010
WP7: β-GLUCAN AND FIBRE ANALYSIS
Daniela Sgrulletta
Sivia Bellato
Viviana Del Frate
Rita Redaelli
AVEQ final Meeting – Bucarest, 18-22 October 2010
MATERIALS & ANALYSES
3 locations each year All the genotypes: total β-glucan
(P2)
70 selected genotypes: soluble β-glucan (P6)fibre & starch (P12)
AVEQ final Meeting – Bucarest, 18-22 October 2010
No. entries Fibre & Starch
Total β-glucan
Soluble β-glucan
210 925 210
ESTONIA (North)
Standard cultivars 62
Selected genotypes 70 70 70 70
Accessions 256 256
388 326
POLAND (Centre)
Standard cultivars 55
Selected genotypes 70 70 70 70
Accessions 259 259
384 329
BULGARIA (South)
Standard cultivars 55
Selected genotypes 70 70 70 70
Accessions 247 247
372 317
PROPOSED ANALYSES - 2008
SELECTED ACCESSIONS – LIST OF THE SPECIES
AVEQ final Meeting – Bucarest, 18-22 October 2010
SPECIES No. of genotypes
SPECIES No. of genotypes
A. abyssinica 1 A. sativa ssp. 29
A. barbata 1 A. sativa var. 2
A. byzantina 2 A. sterilis 1
A. damascena 1 A. strigosa 3
A. fatua 1 A. strigosa ssp.
1
A. hirtula 1 A. strigosa var.
1
A. sativa 25 A. wiestii 1
AVEQ final Meeting – Bucarest, 18-22 October 2010
No. entries Fibre & Starch
Total β-glucan
Soluble β-glucan
ESTONIA (North)
Standard cultivars 62 53 53 53
Selected genotypes 70 63 59 59
Accessions 256
388 116 112 112
POLAND (Centre)
Standard cultivars 55
Selected genotypes 70 2 2 2
Accessions 259 4 4 4
384 6 6 6
BULGARIA (South)
Standard cultivars 55 ON GOING ON GOING
Selected genotypes 70 60 59 59
Accessions 247
372 60 59 59
ANALYSES CARRIED OUT - 2008
AVEQ final Meeting – Bucarest, 18-22 October 2010
METHOD FOR ANALYSIS
Total -glucan content was determined using the enzymatic method of McCleary and Codd (1991).
The same procedure was carried out for the insoluble fraction of -glucan after extraction for 120 min with distilled water at 38°C.
The soluble fraction was calculated for difference (total minus insoluble -glucan), following the indications by Åman and Graham (1987).
AVEQ final Meeting – Bucarest, 18-22 October 2010
Total Insoluble Soluble % Soluble
Estonia Mean 3.97 1.29 2.68 67.12
(59) Min value 2.36 0.57 1.17 47.60
Max value 5.23 2.19 4.25 82.38
St.Dev. 0.56 0.33 0.58 8.51
Bulgaria Mean 4.25 1.56 2.69 63.13
(59) Min value 3.08 0.98 1.64 48.07
Max value 5.36 2.32 3.71 78.49
St.Dev. 0.51 0.33 0.47 6.96
β-GLUCAN CONTENT (% d.m.)
AVEQ final Meeting – Bucarest, 18-22 October 2010
ESTONIA 2008
Total, insoluble and soluble B-glucan (%d.m.) in oat genotypes
0.000
1.000
2.000
3.000
4.000
5.000
6.000
0 10 20 30 40 50 60
Total Insoluble Soluble
AVEQ final Meeting – Bucarest, 18-22 October 2010
BULGARIA 2008
Total, insoluble and soluble B-glucan (%d.m.) in oat genotypes
0.000
1.000
2.000
3.000
4.000
5.000
6.000
7.000
8.000
0 10 20 30 40 50 60
Total Insoluble Soluble
AVEQ final Meeting – Bucarest, 18-22 October 2010
DF Mean Square
Genotype 51 0.71 **
Location 1 4.17 *
GxL 51 0.31 **
Error 102 0.003
ANOVA ANALYSIS
Trials: Bulgaria and Estonia 2008, 52 genotypes
AVEQ final Meeting – Bucarest, 18-22 October 2010
Total Insoluble Soluble % Soluble
AVE 1426 A. hybrida 4.27 0.84 3.43 80.33
AVE 1758 A. fatua 3.45 0.79 2.66 77.10
AVE 2671 A. fatua 3.84 0.68 3.16 82.29
AVE 2781 A. wiestii 6.76 1.53 5.23 77.37
AVE 2804 A. fatua 3.66 0.65 3.01 82.24
PL 52345 A. damascena
6.77 1.48 5.29 78.14
Mean 4.79 1.00 3.80 79.58
St.Dev. 1.55 0.40 1.16 2.37
β-GLUCAN CONTENT IN 6 WILD SPECIES
AVEQ final Meeting – Bucarest, 18-22 October 2010
β-GLUCAN CONTENT IN STANDARD CULTIVARS
ESTONIAHolder FAO CODE
Cultivar Total β-glucan
Holder FAO CODE
Cultivar Total β-glucan
ITA037 Argentina 3.41 EST001 Jaak 3.15
FRA040 Auteuil 4.44 POL054 Krezus 3.70
SWE006 Belinda 4.08 BGR001 Mina 4.38
FRA261 Evora 3.50 ROM007 Mures 3.81
ITA037 Genziana 3.53 CZE047 Saul 3.41
DEU087 Ivory 2.92
Mean 3.67 ± 0.48
LSD 0.38
AVEQ final Meeting – Bucarest, 18-22 October 2010
NIRS CALIBRATION
AVEQ final Meeting – Bucarest, 18-22 October 2010
Statistical data of total -glucan (%as is) NIRS (Near Infrared Spectroscopy) calibration
Calibration setSample number
Mean value
Min value
Max value
RSQ SECV
248 3.51 2.17 6.02 0.793 0.243
Validation set48 3.62 2.65 4.54 0.559 0.319
