Anesthetized Microelectrode Mapping

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Transcript of Anesthetized Microelectrode Mapping

  • 1. Michael P. Kilgard Sensory Experience and Cortical Plasticity University of Texas at Dallas

2. 2010 vs. 7520 V 8119 vs. 3720 V 0 50 100 150 200 250 Week 1 Amplitude(mV) Time (ms) 0 50 100 150 200 250 Week 2 Time (ms) 0 50 100 150 200 250 Week 5 Time (ms) 0 50 100 150 200 250 Week 12 Time (ms) .10 .05 0 -.05 -.10 Red Group Enriched Blue Enriched Environmental Enrichment 22 rats total 3. 40% increase in response strength 1.4 vs. 1.0 spikes per noise burst (p< 0.0001) 10% decrease in frequency bandwidth 2.0 vs. 2.2 octaves at 40dB above threshold (p< 0.05) Three decibel decrease in threshold 17 vs. 20 dB ms (p< 0.001) 1 2 4 8 16 32 0 20 40 60 80 Frequency (kHz) Intensity(dBSPL) A. 1 2 4 8 16 32 0 20 40 60 80 Frequency (kHz) Intensity(dBSPL) B. 0 10 20 30 40 0 50 100 C. Time (ms) Spikes/s 1 2 4 8 16 32 0 20 40 60 80 Frequency (kHz) Intensity(dBSPL) A. 1 2 4 8 16 32 0 20 40 60 80 Frequency (kHz) Intensity(dBSPL) B. Enriched Standard A1 Enrichment Effects - after 2 months N = 16 rats, 820 sites Stronger, More Selective, and More Sensitive Environmental Enrichment Improves Response Strength, Threshold, Selectivity, and Latency of Auditory Cortex Neurons Engineer ND, Percaccio CR, Pandya PK, Moucha R, Rathbun DL, Kilgard MP. Journal of Neurophysiology, 2004. 4. High Low Cochlea Cortex 5. High Low Cochlea Cortex Cortical Map Plasticity 6. High-density microelectrode mapping technique 7. Best Frequency Nucleus Basalis Activity Enables Cortical Map Reorganization M.P. Kilgard, M.M. Merzenich, Science 279(5357): 1714-1718, 1998. download file 8. Tone Frequency - kHz Nucleus Basalis Stimulation Generates Frequency-Specific Map Plasticity N = 20 rats; 1,060 A1 sites 9. Differences between A1 and Posterior Auditory Field submitted 10. 0 20 40 2 4 8 16 32 10 30 50 70 Intensity(dB) -20 -10 0 10 20 2 4 8 16 32 10 30 50 70 Difference in PAF Percent after 19 kHz Paired Intensity(dB) Tone Frequency (kHz) C High frequency map expansion , p