Analyte Calibration Range LOD LOQ
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Table 1. Data for LOD and LOQ of Anthocyanins Analyte Calibration Range (μg/mL) LOD (μg/mL) LOQ (μg/mL) Dp3Glu 0.39–50 1.56 6.25 Cy3Gal 0.39–25 0.78 3.13 Cy3Glu 0.39–25 0.78 3.13 Pet3Glu 0.39–25 0.20 0.78 Peo3Gal 0.39–10 0.56 2.34 Peo3Ara 0.39–25 0.78 3.13 Mal3Gal 0.39–25 0.20 0.78 Table 2. Determination of Anthocyanins in NIST and USP Samples Analyte NIST Sample (μg/mL) USP Sample (μg/mL) Dp3Glu 14.9 12.2 Cy3Gal 8.01 7.00 Cy3Glu 7.59 6.61 Pet3Glu 6.08 5.14 Peo3Ara 5.76 5.22 Peo3Gal 1.53 1.45 Mal3Gal 2.58 2.24 Table 3. Recovery of Anthocyanins in Different Bilberry-Based Products Sample Analytes Amount Spiked (µg/mL) % Recovery Eye Antioxidant Dp3Glu 10 89.3 Cy3Gal 3.0 94.8 Cy3Glu 3.0 81.1 Peo3Gal 0.5 96.2 Pet3Glu 3.0 92.6 Mal3Gal 0.5 94.2 Peo3Ara 3.0 91.2 Brand A Bilberry- Based Nutritional Supplement Dp3Glu 25 90.2 Cy3Gal 10 82.8 Cy3Glu 10 78.7 Peo3Gal 4.0 98.1 Pet3Glu 10 78.4 Mal3Gal 10 107 Peo3Ara 10 104 Brand B Bilberry- Based Nutritional Supplement Dp3Glu 20 97.6 Cy3Gal 10 84.0 Cy3Glu 10 93.6 Peo3Gal 2.0 105 Pet3Glu 10 83.7 Mal3Gal 3.0 101 Peo3Ara 5.0 108 28820 R3 R5 R3 Anthocyanin H H Glucose/Arabinose/Galactose Pelargonidin 3-Glucose/Arabinose/ Galactose OH H Glucose/Arabinose/Galactose Cyanidin 3-Glucose/Arabinose/Galactose OH OH Glucose/Arabinose/Galactose Delphinidin 3-Glucose/Arabinose/ Galactose OCH 3 OH Glucose/Arabinose/Galactose Petunidin 3-Glucose/Arabinose/ Galactose OCH 3 H Glucose/Arabinose/Galactose Peonidin 3-Glucose/Arabinose/Galactose OCH 3 OCH 3 Glucose/Arabinose/Galactose Malvidin 3-Glucose/Arabinose/Galactose OH HO 0-R3 OH R3' R5' O + Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 20.1 3. Cy3Gal 11.0 4. Dp3Ara — 5. Cy3Glu 12.3 6. Pet3Gal — 7. Cy3Ara — 8. Dp — 9. Pet3Glu 9.91 10. Peo3Gal 3.91 11. Pet3Ara — 12. Peo3Glu — 13. Mal3Gal 8.76 14. Peo3Ara 12.7 15. Cy — 16. Mal3Glu — 17. Mal3Ara — 18. Pet — 19. Peo — 20. Mal — 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 20 19 18 17 16 15 14 13 12 11 9 8 7 6 5 4 3 1 2 -0.5 0 8 Minutes mAU Column: Acclaim 120, C18, 2.2 μm, analytical (2.1 × 150 mm) Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrile Gradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B Hold for 5 min at 9% B Temp.: 35 °C Flow: 0.475 mL/min Detection: Absorbance, Vis 520 nm Inj. Volume: 2.0 μL Sample: 125 μg/mL15 Anthocyanin standard 29008 10 29009 Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 5.15 3. Cy3Gal 2.23 4. Dp3Ara — 5. Cy3Glu 2.02 6. Pet3Gal — 7. Cy3Ara — 8. Pet3Glu 1.89 9. Peo3Gal 0.28 10. Pet3Ara — 11. Mal3Gal 4.27 12. Peo3Ara 1.49 13. Cy — 14. Mal3Glu — Column: Acclaim 120, C18, 2.2 μm, analytical (2.1 × 150 mm) Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrile Gradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B Hold for 5 min at 9% B Temp.: 35 °C Flow: 0.475 mL/min Detection: Absorbance, Vis 520 nm Inj. Volume: 2.0 μL Sample: Eye antioxidant 0 2 4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 6 8 10 12 14 16 18 20 22 24 26 28 30 -0.1 0 1.1 Minutes mAU 29010 Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 21.7 3. Cy3Gal 11.9 4. Dp3Ara — 5. Cy3Glu 10.6 6. Pet3Gal — 7. Cy3Ara — 8. Dp — 9. Pet3Glu 8.76 10. Peo3Gal 3.31 11. Pet3Ara — 12. Peo3Glu — 13. Mal3Gal 6.26 14. Peo3Ara 8.88 15. Cy — 16. Mal3Glu — 17. Mal3Ara — 18. Pet — 19. Peo — 20. Mal — Column: Acclaim 120, C18, 2.2 μm, analytical (2.1 × 150 mm) Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrile Gradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B Hold for 5 min at 9% B Temp.: 35 °C Flow: 0.475 mL/min Detection: Absorbance, Vis 520 nm Inj. Volume: 2.0 μL Sample: Nutritional supplement A 0 1 2 3 4 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 -1 0 12 5 6 7 8 10 11 12 13 15 14 16 17 18 19 9 20 Minutes mAU 29011 Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 20.8 3. Cy3Gal 10.2 4. Dp3Ara — 5. Cy3Glu 9.99 6. Pet3Gal — 7. Cy3Ara — 8. Delphinidin — 9. Pet3Glu 8.40 10. Peo3Gal 1.12 11. Pet3Ara — 12. Peo3Glu — 13. Mal3Gal 3.27 14. Peo3Ara 6.96 15. Cyanidin — 16. Mal3Glu — 17. Mal3Ara — 18. Petunidin — 19. Peonidin — 20. Malvidin — Column: Acclaim 120, C18, 2.2 μm, analytical (2.1 × 150 mm) Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrile Gradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B 30.0–35.0 min 9% B Temp.: 35 °C Flow: 0.475 mL/min Detection: Absorbance, Vis 520 nm Inj. Volume: 2.0 μL Sample: Nutritional supplement B 0 2 1 2 3 4 5 6 7 8 10 11 12 13 14 16 17 18 19 20 15 9 4 6 8 10 12 14 16 18 20 22 24 26 28 30 -0.5 0 7 Minutes mAU
Transcript of Analyte Calibration Range LOD LOQ
Table 1. Data for LOD and LOQ of Anthocyanins
Analyte Calibration Range (μg/mL)
LOD (μg/mL)
LOQ (μg/mL)
Dp3Glu 0.39–50 1.56 6.25Cy3Gal 0.39–25 0.78 3.13Cy3Glu 0.39–25 0.78 3.13Pet3Glu 0.39–25 0.20 0.78Peo3Gal 0.39–10 0.56 2.34Peo3Ara 0.39–25 0.78 3.13Mal3Gal 0.39–25 0.20 0.78
Table 2. Determination of Anthocyanins in NIST and USP Samples
Analyte NIST Sample (μg/mL) USP Sample (μg/mL)Dp3Glu 14.9 12.2Cy3Gal 8.01 7.00 Cy3Glu 7.59 6.61 Pet3Glu 6.08 5.14 Peo3Ara 5.76 5.22 Peo3Gal 1.53 1.45 Mal3Gal 2.58 2.24
Table 3. Recovery of Anthocyanins in Different Bilberry-Based Products
Sample Analytes Amount Spiked (µg/mL) % Recovery
Eye Antioxidant
Dp3Glu 10 89.3Cy3Gal 3.0 94.8Cy3Glu 3.0 81.1
Peo3Gal 0.5 96.2Pet3Glu 3.0 92.6Mal3Gal 0.5 94.2Peo3Ara 3.0 91.2
Brand A Bilberry-Based Nutritional
Supplement
Dp3Glu 25 90.2Cy3Gal 10 82.8Cy3Glu 10 78.7
Peo3Gal 4.0 98.1Pet3Glu 10 78.4Mal3Gal 10 107Peo3Ara 10 104
Brand B Bilberry-Based Nutritional
Supplement
Dp3Glu 20 97.6Cy3Gal 10 84.0Cy3Glu 10 93.6
Peo3Gal 2.0 105Pet3Glu 10 83.7Mal3Gal 3.0 101Peo3Ara 5.0 108
28820
R3 R5 R3 Anthocyanin
H H Glucose/Arabinose/Galactose Pelargonidin 3-Glucose/Arabinose/Galactose
OH H Glucose/Arabinose/Galactose Cyanidin 3-Glucose/Arabinose/Galactose
OH OH Glucose/Arabinose/Galactose Delphinidin 3-Glucose/Arabinose/Galactose
OCH3 OH Glucose/Arabinose/Galactose Petunidin 3-Glucose/Arabinose/Galactose
OCH3 H Glucose/Arabinose/Galactose Peonidin 3-Glucose/Arabinose/Galactose
OCH3 OCH3 Glucose/Arabinose/Galactose Malvidin 3-Glucose/Arabinose/Galactose
OH
HO
0-R3
OH
R3'
R5'
O+
Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 20.1 3. Cy3Gal 11.0 4. Dp3Ara — 5. Cy3Glu 12.3 6. Pet3Gal — 7. Cy3Ara — 8. Dp — 9. Pet3Glu 9.91 10. Peo3Gal 3.91 11. Pet3Ara — 12. Peo3Glu — 13. Mal3Gal 8.76 14. Peo3Ara 12.7 15. Cy — 16. Mal3Glu — 17. Mal3Ara — 18. Pet — 19. Peo — 20. Mal —
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Column: Acclaim 120, C18, 2.2 µm, analytical (2.1 × 150 mm)Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrileGradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B Hold for 5 min at 9% BTemp.: 35 °CFlow: 0.475 mL/minDetection: Absorbance, Vis 520 nmInj. Volume: 2.0 µLSample: 125 µg/mL15 Anthocyanin standard
29008
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29009
Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 5.15 3. Cy3Gal 2.23 4. Dp3Ara — 5. Cy3Glu 2.02 6. Pet3Gal — 7. Cy3Ara — 8. Pet3Glu 1.89 9. Peo3Gal 0.28 10. Pet3Ara — 11. Mal3Gal 4.27 12. Peo3Ara 1.49 13. Cy — 14. Mal3Glu —
Column: Acclaim 120, C18, 2.2 µm, analytical (2.1 × 150 mm)Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrileGradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B Hold for 5 min at 9% BTemp.: 35 °CFlow: 0.475 mL/minDetection: Absorbance, Vis 520 nmInj. Volume: 2.0 µLSample: Eye antioxidant
0 2 4
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Minutes
mAU
29010
Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 21.7 3. Cy3Gal 11.9 4. Dp3Ara — 5. Cy3Glu 10.6 6. Pet3Gal — 7. Cy3Ara — 8. Dp — 9. Pet3Glu 8.76 10. Peo3Gal 3.31 11. Pet3Ara — 12. Peo3Glu — 13. Mal3Gal 6.26 14. Peo3Ara 8.88 15. Cy — 16. Mal3Glu — 17. Mal3Ara — 18. Pet — 19. Peo — 20. Mal —
Column: Acclaim 120, C18, 2.2 µm, analytical (2.1 × 150 mm)Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrileGradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B Hold for 5 min at 9% BTemp.: 35 °CFlow: 0.475 mL/minDetection: Absorbance, Vis 520 nmInj. Volume: 2.0 µLSample: Nutritional supplement A
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Peaks: Conc. (μg/mL) 1. Dp3Gal — 2. Dp3Glu 20.8 3. Cy3Gal 10.2 4. Dp3Ara — 5. Cy3Glu 9.99 6. Pet3Gal — 7. Cy3Ara — 8. Delphinidin — 9. Pet3Glu 8.40 10. Peo3Gal 1.12 11. Pet3Ara — 12. Peo3Glu — 13. Mal3Gal 3.27 14. Peo3Ara 6.96 15. Cyanidin — 16. Mal3Glu — 17. Mal3Ara — 18. Petunidin — 19. Peonidin — 20. Malvidin —
Column: Acclaim 120, C18, 2.2 µm, analytical (2.1 × 150 mm)Eluent: A: 10% Formic acid B: 10% Formic acid, 22.5% methanol, 22.5% acetonitrileGradient: 0.0–12.0 min 9% B 12.0–25.0 min 35% B 25.0–30.0 min 50% B 30.0–35.0 min 9% BTemp.: 35 °CFlow: 0.475 mL/minDetection: Absorbance, Vis 520 nmInj. Volume: 2.0 µLSample: Nutritional supplement B
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