AMPK Signaling: The Fuel Sensor and Regulator...

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AMPK-α(1,2) AMPK-β(1,2) AMPK-γ(1,2,3) TAK1 Leptin AMP/ATP LKB1 ATM Akt IL-6 Insulin PP2C CaMKK Ca 2+ Adiponectin Lipolysis Fatty Acid Synthesis Cholesterol Synthesis Fatty Acid Oxidation Fatty Acid Uptake HSL HMG-CoA Reductase PEPCK G6Pase AS160 TBC1D1 MEF2A MEF2D Malonyl-CoA SREBP-1C ACC1 ACC2 CPT-1 MCD LPL CD36 Glycogen Synthesis GLUT4 Biogenesis GLUT4 Translocation Glucose Uptake Glycolysis Gluconeogenesis GSK-3 GS PFK2 NO Synthesis Energy Expenditure eNOS NAD + / NADH TSC1/ TSC2 4EBP1 eIF4E SIRT1 Protein Translation Initiation Peptide Elongation Mitochondrial Biogenesis Protein Translation eEF2K eEF2 mTOR RheB p70S6K PGC-1α S6 NRF-1 AICAR Exercise Discover more at abcam.com/AMPKsignaling AMPK Signaling: The Fuel Sensor and Regulator Pathway Produced in collaboration with Ding An, Ph.D., Postdoctoral Fellow and Laurie J Goodyear, Ph.D., Associate Professor of Medicine, Harvard Medical School & Senior Investigator and Head, Section on Metabolism, Joslin Diabetes Center. Legend: Regulation of AMPK Lipid Metabolism Carbohydrate Metabolism Protein Synthesis Others Activation Activation (not fully confirmed) Inhibition

Transcript of AMPK Signaling: The Fuel Sensor and Regulator...

AMPK-α(1,2)

AMPK-β(1,2) AMPK-γ(1,2,3)

TAK1

Leptin

AMP/ATP LKB1 ATM Akt

IL-6Insulin

PP2CCaMKKCa2+

Adiponectin

Lipolysis

Fatty AcidSynthesis

CholesterolSynthesis

Fatty AcidOxidation

Fatty AcidUptake

HSL

HMG-CoAReductase

PEPCKG6Pase

AS160TBC1D1

MEF2AMEF2D

Malonyl-CoA

SREBP-1C

ACC1

ACC2

CPT-1

MCD

LPLCD36

GlycogenSynthesis

GLUT4Biogenesis

GLUT4Translocation

GlucoseUptakeGlycolysis

Gluconeogenesis

GSK-3

GS

PFK2NO

Synthesis

EnergyExpenditure

eNOS

NAD+/NADH

TSC1/TSC2

4EBP1eIF4E

SIRT1

ProteinTranslationInitiation

PeptideElongation

MitochondrialBiogenesis

ProteinTranslation

eEF2K

eEF2

mTORRheB

p70S6KPGC-1α

S6

NRF-1

AICARExercise

Discover more at abcam.com/AMPKsignaling

AMPK Signaling: The Fuel Sensor and Regulator Pathway

Produced in collaboration with Ding An, Ph.D., Postdoctoral Fellow and Laurie J Goodyear, Ph.D., Associate Professor of Medicine, Harvard Medical School & Senior Investigator and Head, Section on Metabolism, Joslin Diabetes Center.

Legend:

Regulation of AMPK

Lipid Metabolism

Carbohydrate Metabolism

Protein Synthesis

Others

Activation

Activation (not fully confirmed)

Inhibition

Discover more at abcam.com/AMPKsignaling

081-09/09-PW

Copyright © 2013 Abcam. All Rights Reserved.

AMPK Signaling: The fuel sensor and regulator Pathway

Antibody Clonality Applications Host Species Reactivity Product Code

Adiponectin M IHC-P, WB, ELISA, ICC/IF Ms Hu, Ms, Bb ab22554

Akt1 protein (tagged) WB Hu ab43619

Akt2 protein Inhib Hu ab42593

Akt3 protein (active) SDS-PAGE, FuncS Hu ab79799

AMPK alpha 1 M IHC-P, IP, WB Rb Hu, Ms, Rat ab32047

AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) P ELISA, IHC-Fr, IHC-P, WB Rb Hu, Ms, Rat ab39400AMPK alpha 1 + AMPK beta 1 + AMPK gamma 1 protein (active) FuncS, SDS-PAGE Hu ab79803

AMPK alpha 1 peptide BL Hu ab40461

AMPK alpha 2 P IP, WB Rb Hu, Ms, Rat, Cow ab3760

AMPK alpha 2 peptide BL Hu ab40462

AMPK beta 1 M Flow Cyt, IHC-P, IP, WB Rb Hu, Ms, Rat ab32112

AMPK gamma 1 M Flow Cyt, ICC, IHC-P, IP, WB Rb Hu ab32382

ATM M ICC/IF, IHC-P, IP, WB Rb Hu ab32420

CaMKK M IHC-P, WB Ms Hu ab57913

CaMKK protein (tagged) SDS-PAGE, FuncS Hu ab55692

CD36 M BL, Flow Cyt, IHC-Fr, IP, WB Ms Hu, Rat ab17044

CPT1A P WB, ELISA Gt Hu, Predicted: Chmp, RMk ab53532

EEF2K protein (active) SDS-PAGE, FuncS, WB Hu ab84570

eIF4E P ICC/IF, IP, WB Rb Hu ab1126

eNOS P IHC-P Ms Hu, Predicted: Ms, Rat, Cow, Dog, Pig ab15280

GLUT4 P IHC-P, WB Rb Hu, Predicted: Ms, Rat, Cow, Goat, Hrs, Rb, Shp ab33780

HMG-CoA reductase / HMGCR (phospho S872) P WB Rb Hu, Ms, Predicted: Rat ab78275

HSL / LIPE P WB, ELISA Chk Hu, Ms, Rat ab17652

Insulin protein (active) SDS-PAGE, FuncS Hu ab73797

Leptin protein WB, ELISA Hu ab13987

LKB1 M IHC-P, WB, ELISA, ICC/IF Ms Hu ab15095

MEF2A P ELISA, ICC/IF, IHC-P, WB Rb Hu, Ms, Rat ab51153

mTOR P ICC, IHC-Fr, IP, WB Rb Hu, Ms, Rat ab2732

NRF1 M IHC-P, WB Ms Hu ab55744

PEPCK1 / PCK1 P IHC-P, WB Rb Hu, Rat ab28455

PFK2 / PFKFB2 P WB, ELISA Rb Hu ab70175

PGC1 alpha P IHC-P, WB, ELISA Rb Hu, Ms, Predicted: Rat, Chk, Cow, CynMk, Dog, Hrs, Op, Pig, RMk

ab54481

S6K protein (active) SDS-PAGE, FuncS Hu ab62291

SIRT1 M IHC-P, ICC, WB, IP Rb Hu ab32441

TAK1 P ELISA, ICC/IF, IHC-P, WB Rb Hu, Predicted: Ms, Rat ab79354

TBC1D1 P WB Rb Hu, Ms ab84692

Related antibodiesAMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) (ab39400)Clonality Applications P ELISA, IHC-Fr, IHC-P, WBHost Species cross reactivity Rb Hu, Ms, Rat

ATM (ab32420)Clonality Applications M ICC/IF, IHC-P, IP, WBHost Species cross reactivity Rb Hu

MEF2A (ab51153)Clonality Applications P ELISA, ICC/IF, IHC-P, WBHost Species cross reactivity Rb Hu, Ms, Rat

mTOR (ab2732)Clonality Applications P ICC, IHC-Fr, IP, WBHost Species cross reactivity Rb Hu, Ms, Rat

SIRT1 (ab32441)Clonality Applications M IHC-P, ICC, WB, IP Host Species cross reactivity Rb Hu

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