Alan Dorsinville, Reading High School Natalie Gibbs, Reading High School

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Transcript of Alan Dorsinville, Reading High School Natalie Gibbs, Reading High School

  • Alan Dorsinville, Reading High SchoolNatalie Gibbs, Reading High School

  • IntroductionThe Problem and our solutionBackground InformationThe purpose of PADsMaterialsProcedureScience ConceptsResultsTeam F(Floral Experiment)Conclusion/DiscussionFurther ResearchAcknowledgements

  • The cost of health care is an issue in AmericaTesting requiresTimeMoneyInsuranceTechnical ExperienceEtcAll of which is inconvenientDeveloping Countries

  • Micro fluidic paper-based assay devices (PAD)A paper diagnostic testPaper-based devices areInexpensiveQuickEasy to useRequire a small volume of liquidLack the use of advanced equipmentEffective and accurate

  • Your body has many substances including proteins and glucoseGlucose provides energy for your body an all of your movements

    Glucose ConcentrationDisease0-0.8mMNormalAbove 0.8mMImpaired kidney and/or diabetes

  • Proteins are important for growth, tissue repair, and many other bodily functions

  • Our purpose is to show we can quantify diagnostic results using a cheap paper deviceOur chips are designed to detect glucose and protein in our substances

  • Intermolecular forces

    Capillary actionAllows us to direct small amounts of liquid to testing wells

  • CleWinChromatography paper( Whatman)PrinterScales, beakers, pipettesVarious chemicalsInfrared GunHot PlateScannerAdobe Photoshop

  • Part One: PlanningCleWin is a computer program made to design our chips

  • Step 1: PrintingThe pattern is outlined with wax when printedStep 2: Place the chips on a hot plate at 150CAllows wax to seep through

  • This project requires making both a protein and glucose reagentA chemical reagent is a substance used in a chemical reaction to detect, measure, examine, or produce other substances

  • Buffer (pH=6.0)0.2 M NaH2PO40.2 M Na2HPO40.3 M Trehalose0.6 M KI30 units/mL HRP120 units/mL GO

  • Glucose + Glucose oxidase Gluconic acid + Hydrogen peroxide (H2O2)

    H2O2H2O + O2

    I- I2 HR PeroxidaseBrown color

  • Part 1:0.25 M Citric acid (pH 1.8 buffer)184 L H2O, 16 L EtOHPart 2:9 mM TBPB10 L H2O, 190 L EtOHProtein Mechanism TBPB + protein = Blue color

  • Apply 0.2 L of reagents using a micropipette. Must wait ten minutes

    Part Five: Test One

  • We made a new design for our second chip on CleWin, printed them, and reapplied the chemical reagents, etc.

  • The chips are a urine analysis test so we made an artificial urine sampleWe made several concentrations glucose and proteins to test

  • We performed eight tests for each of the eleven different concentrations of glucose and proteinAfter 30 minutes, the chips were scanned into the computer

  • Team Fs research is focused on the relationship between pollinators and nectar

  • We made adjustments to our second chip to create a more effective testWe were not able to quantify, but still proved the chips had the potential to quantify different detectable substancesWe were able to rank the glucose concentration of Team Fs nectar solutions

  • The PADs serve the same purpose as other urine testsScientists are trying to find ways of detecting more diseases with the paper-based analytical device

  • We would like to thank:Dr. Scott PhillipsSeeCos FacultyChris DalyMs. Jody MarkleyMr. Derek JamesMs. Jean Marie DonnellyUBMS Staff

  • Briefly describe shape and what it does********Put pics from first test to come up or video**We made eleven various concentrations of glucose and protein out of the urine sampleV1=m2 x v2 m1

    *We made the stems widerWe made four branchesWe used triangles for all our wells

    *Must research how urine tests work now*