AhR
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AhR siRNA C AhR β- tubulin -- - -- - -- - + - + --- +++ -- - + - + +++ -- - --+ - -+ Buffer rVpr Cont. AhR siRNA A -- - -- - -- - + - + --- +++ -- - + - + +++ -- - --+ - -+ Buffer rVpr Cont. ARNT1 siRNA β-actin β-actin U β- tubulin ARNT1 siRNA U C ARNT1 B C D β- tubulin C/EBP-β U C ① ② siRNA E 12345 67 89 Mr 12345 6789 Mr U C CREB siRNA β-tubulin CREB ①②③①②③ β- tubulin c-Jun U C siRNA ①②③①②③ c-Jun F G Supplementary Figure S8. Effects of siRNAs for AhR, ARNT1 CREB, and c-Jun on expression of endogenous proteins. A, C, E, F, G. Effective down-regulation of endogenous proteins by siRNAs. AhR, ARNT1, C/EBPβ, CREB and c-Jun siRNA were transfected into HuH-7. Untreated cell (U); control siRNA (C). B, D. Effect of AhR, ARNT1, siRNA on the L1-RTP. Control siRNA (Cont.) was also included. Different siRNAs of AhR and ARNT1 were used in the experiments shown in Figure 5. rVpr induced L1-RTP required AhR but not ARNT1. Supplementary Figure S8
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Supplementary Figure S8. B. A. +. -. -. -. -. -. +. +. -. Buffer. +. +. -. -. -. -. +. -. -. rVpr. siRNA. Cont. -. +. +. +. -. -. -. -. -. siRNA. U. +. -. -. -. +. -. -. -. +. AhR. C. AhR. AhR. β-tubulin. β-actin. 1. 2. 3. Mr. 4. 5. 6. 7. - PowerPoint PPT Presentation
Transcript of AhR
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Supplementary Figure S8. Effects of siRNAs for AhR, ARNT1 CREB, and c-Jun on expression of endogenous proteins. A, C, E, F, G. Effective down-regulation of endogenous proteins by siRNAs. AhR, ARNT1, C/EBPβ, CREB and c-Jun siRNA were transfected into HuH-7. Untreated cell (U); control siRNA (C). B, D. Effect of AhR, ARNT1, siRNA on the L1-RTP. Control siRNA (Cont.) was also included. Different siRNAs of AhR and ARNT1 were used in the experiments shown in Figure 5. rVpr induced L1-RTP required AhR but not ARNT1.
Supplementary Figure S8
