across the Blood-Brain Barrier and Inhibitors of β …across the Blood-Brain Barrier and Inhibitors...

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1 Nontoxic Amphiphilic Carbon Dots as Promising Drug Nanocarriers across the Blood-Brain Barrier and Inhibitors of β-Amyloid Yiqun Zhou, 1 Piumi Y. Liyanage, 1 Dinesh Devadoss, 2 Linda Rebeca Rios Guevara, 1 Ling Cheng, 1 Regina M. Graham, 3 Hitendra S. Chand, 2 Abdulrahman O. Al-Youbi, 4 Abdulaziz S. Bashammakh, 4 Mohammad S. El-Shahawi, 4 Roger M. Leblanc 1 * 1 Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA 2 Department of Immunology and Nano-Medicine, Florida International University, Miami, FL 33199, USA 3 Department of Neurological Surgery, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA 4 Department of Chemistry, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Kingdom of Saudi Arabia Fig. S1. Confocal images of Y-CDs aqueous dispersion (0.1 mg/mL) permeated into the blood (a, b) and CNS: spinal cord (c, d) and brain (e, f) of zebrafish. (a, c, e are merged images of bright field and under the excitation of 405 nm; b, d, f are images under the excitation of 405 nm only). The figure is adapted with the permission from the publisher. 1 Fig. S2. Confocal images of zebrafish soaked in different carbon dots solutions (0.1 mg/mL). (left- right: control (a), carbon dots from carbon nanopowder (b), and from BSA (c)). Electronic Supplementary Material (ESI) for Nanoscale. This journal is © The Royal Society of Chemistry 2019

Transcript of across the Blood-Brain Barrier and Inhibitors of β …across the Blood-Brain Barrier and Inhibitors...

Page 1: across the Blood-Brain Barrier and Inhibitors of β …across the Blood-Brain Barrier and Inhibitors of β-Amyloid Yiqun Zhou,1 Piumi Y. Liyanage,1 Dinesh Devadoss,2 Linda Rebeca Rios

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Nontoxic Amphiphilic Carbon Dots as Promising Drug Nanocarriers across the Blood-Brain Barrier and Inhibitors of β-Amyloid Yiqun Zhou,1 Piumi Y. Liyanage,1 Dinesh Devadoss,2 Linda Rebeca Rios Guevara,1 Ling Cheng,1 Regina M. Graham,3 Hitendra S. Chand,2 Abdulrahman O. Al-Youbi,4 Abdulaziz S. Bashammakh,4 Mohammad S. El-Shahawi,4 Roger M. Leblanc1*1 Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA2 Department of Immunology and Nano-Medicine, Florida International University, Miami, FL 33199, USA3 Department of Neurological Surgery, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA4 Department of Chemistry, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Kingdom of Saudi Arabia

Fig. S1. Confocal images of Y-CDs aqueous dispersion (0.1 mg/mL) permeated into the blood (a, b) and CNS: spinal cord (c, d) and brain (e, f) of zebrafish. (a, c, e are merged images of bright field and under the excitation of 405 nm; b, d, f are images under the excitation of 405 nm only). The figure is adapted with the permission from the publisher.1

Fig. S2. Confocal images of zebrafish soaked in different carbon dots solutions (0.1 mg/mL). (left-right: control (a), carbon dots from carbon nanopowder (b), and from BSA (c)).

Electronic Supplementary Material (ESI) for Nanoscale.This journal is © The Royal Society of Chemistry 2019

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Fig. S3. TEM images of other two CDs aqueous dispersions (0.1 mg/mL). (a) CDs prepared from carbon nanopowder; (b) CDs prepared from BSA.

(a)

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(b)

Fig. S4. The fluorescence emission spectra of Y-CDs coated with 3-amino-1-propanol (a) or DEA (c) measured with a 1 cm quartz cuvette; FTIR spectra of Y-CDs coated with 3-amino-1-propanol (b) or DEA (d) with air as the background.

400 500 600 700 800 9000

2

4

6

8

10

Inte

nsity

(a.u

.)

Wavelenght (nm)

350 nm 375 400 425 450

4000 3500 3000 2500 2000 150050

60

70

80

90

100

110

Tran

smitt

ance

(%)

Wavenumber (cm-1)

3-amino-1-propanol Y-CDs Conjugate

1500 cm-1

C-C stretch (in-ring)

1641 cm-1

N-H bend

3300 cm-1

O-H or N-H stretch

2926-2827 cm-1

C-H stretch

(a) (b)

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400 500 600 700 800 9000.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5In

tens

ity (a

.u.)

Wavelength (nm)

350 nm 375 400 425 450

4000 3500 3000 2500 2000 150050

60

70

80

90

100

110

1500 cm-1

C-C stretch (in-ring)

1641 cm-1

N-H bend

3300 cm-1

O-H or N-H stretch

Tran

smitt

ance

(%)

Wavenumber (cm-1)

Diethanolamine Y-CDs Conjugate

2926-2827 cm-1

C-H stretch

(c) (d)

Table 1. Zeta potential values of all the three CDs aqueous dispersions (0.1 mg/mL).

Black CDs Y-CDs BSA CDsZeta potential (mV) -13.9 -15.3 -12.7

Table 2. Particle sizes of all the three types of CDs.

Black CDs Y-CDs BSA CDsMean (nm) 3.34 3.416 3.227

STDEV 0.988 0.985 0.937Minimum (nm) 1.154 1.351 1.538Maximum (nm) 6.401 5.731 6.866

References

1. Y. Zhou, K. J. Mintz, S. K. Sharma and R. M. Leblanc, Langmuir, 2019, 35, 9115-9132.