319.00 (1.00) Fortis Phenyl 150x4.6mm 5µ • p/n : FPH-050705€¦ · 800 1000 1200 6. 84 3 411 88...
13
Strength in Technology PK11195 – PET Tracer • Column : Fortis Phenyl 150x4.6mm 5µ • p/n : FPH-050705 • Mobile Phase: 60: 40 ACN : H2O • Flow : 1ml/min • Temp : • λ : MS Detection 1. De-chlorinated PK11195 2. PK11195 0.0 2.5 5.0 7.5 10.0 12.5 15.0 319.00 (1.00) 353.00 (1.00)
Transcript of 319.00 (1.00) Fortis Phenyl 150x4.6mm 5µ • p/n : FPH-050705€¦ · 800 1000 1200 6. 84 3 411 88...
Strength in Technology
PK11195 – PET Tracer• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
60: 40 ACN : H2O
• Flow : 1ml/min• Temp : • λ : MS Detection
1. De-chlorinated PK111952. PK11195
0.0 2.5 5.0 7.5 10.0 12.5 15.0
319.00 (1.00)353.00 (1.00)
Strength in Technology
Quinidine• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
70:30 H2O + 0.1% Formic acid :MeOH
• Flow : 1ml/min• Temp : 25oC• λ : 235nm
1. Quinidine2. Dihydroquinidine
Minutes0 2 4 6 8 10 12 14 16 18 20
60
80
100
120
140
160
180
Strength in Technology
Stemetil• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
35:65 H2O + 0.1% Formic acid :MeOH
• Flow : 1ml/min• Temp : 25oC• λ : 254nm
1. Prochlorperazine Maleate
Minutes0 1 2 3 4 5 6 7 8 9 10
50
60
70
80
90
Strength in Technology
Vitamin C• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
100% H2O +0.1% Formic acid
• Flow : 0.8ml/min• Temp : 25oC• λ : 254nm
1. Vitamin C
Minutes0 1 2 3 4 5 6 7 8 9 10
0
2
4
6
8
10
12
14
Strength in Technology
Benzodiazepines• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
50 : 50 H2O + 0.1% Formic acid : ACN
• Flow : 1ml/min• Temp : 25oC• λ : 230nm
1. Lorazepam2. Temazepam2. Diazepam
Minutes0 5 10 15 20 25 30
Volts
0.05
0.06
0.07
0.08
Strength in Technology
Ciprofloxacin• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
A – H2O + 0.1% formic acidB – MeOH + 0.1% formic acid
• Gradient: 10-90% in 10mins
• Flow : 1ml/min• Temp : 25oC• λ : 254nm
1. Ciprofloxacin
Minutes0 1 2 3 4 5 6 7 8 9 10
200
400
600
800
1000
1200
6.84
341188
1.30
8.41
00.00
8.74
00.00
9.06
00.00
9.77
00.00
DianetteDianetteColumn : Fortis Phenyl 150x4.6mm 5μp/n : FPH-050705Mobile Phase:
20:80 – 20mM NH4OAc : MeOH
Flow : 1ml/minTemp : 20oCλ : 210nm
1. Dianette
Minutes0 2 4 6 8 10 12 14
50
75
125
150
Strength in Technology
Doxazosin• Column : Fortis Phenyl 150x4.6mm 5µ• p/n : FPH-050705• Mobile Phase:
30:60 H2O + 0.1% Formic acid :MeOH
• Flow : 1ml/min• Temp : 25oC• λ : 254nm
1. Doxazosin
Minutes0.0 1.0 2.0 3.0 4.0 5.0 6.0
60
80
100
120
140
GliclazideGliclazideColumn : Fortis Phenyl 50x2.1mm 2.1μp/n : F2PH-020302Mobile Phase:
80:20 – H2O + 0.1% formic acid:ACN
Flow : 0.4ml/minTemp : 20oCλ : 254nm
1. Gliclazide
Minutes0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
mA
U
0
100
200
300
400
mA
U
0
100
200
300
400
0.38
2 1
.44
216
2
0.68
5 2
.07
0
0.84
3 5
.19
144
759
1.67
5 1
.49
607
30
Positional IsomersPositional IsomersHydroxybenzoic Hydroxybenzoic acidsacids
Column : Fortis Phenyl 150x4.6mm 5μp/n : FPH-050705Mobile Phase:
50:50 – H2O + 0.1% formic acid:MeOH
Flow : 1ml/minTemp : 20oCλ : 280nm
1. 4-Hydroxybenzoic acid2. 3-Hydroxybenzoic acid3. 2-Hydroxybenzoic acid
Minutes0 1 2 3 4 5 6 7 8 9 10
100
200
300
400
500
600
700
OH
OH
O
Positional IsomersPositional IsomersSteriodsSteriods
Column : Fortis Phenyl 150x4.6mm 5μp/n : FPH-050705Mobile Phase:
40 : 60 H2O : MeOH
Flow : 1ml/minTemp : 20oCλ : 210nm
1. 4-Hydroxyestradiol (mw=288.38)
2. 2-Hydroxyestradiol (mw=288.38)
Minutes1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
mA
U
50
75
100
125
150
175
200
mA
U
50
75
100
125
150
175
200OH
OH
CH3
OH
H
H
H
OH
CH3
OH
H
H
H
OH
Positional IsomersPositional IsomersNicotinamidesNicotinamides
Column : Fortis Phenyl 150x4.6mm 5μp/n : FPH-050705Mobile Phase:
80:20 – 20mM NH4OAc :MeOH
Flow : 1ml/minTemp : 20oCλ : 254nm
1. Isonicotinamide2. Nicotinamide
Minutes0 1 2 3 4 5 6 7 8 9 10
50
75
100
125
150
175
200
225
N
NH2
ON
NH2O
Strength in Technology
Paludrine• Column : Fortis Phenyl 50x4.6mm 5µ• p/n : FPH-050305• Mobile Phase:
A – H2O + 0.1% formic acidB – MeOH + 0.1% formic acid
• Gradient: 10-90% in 10mins
• Flow : 1ml/min• Temp : 25oC• λ : 254nm
1. Proguanil
Minutes1 2 3 4 5 6 7 8 9 10
100
200
300
400
500
600
700
800
