1. KUMAR ORGANIC PRODUCTS LIMITEDIngredients for usBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012

2. SODIUMHYALURONATE(KOPURON) Pharma grade BIO/003/2012-13Dr. Lakshmi Sridhar18th Sep 2012 3. Sodium hyaluronate INCI name: Sodium hyaluronate CAS No. 9057-32-7 Product name: Poly-(sodium--D-glucuronate-[1-3}- N-acetyl-D-glucosamine-[1-4] Other names: Hyaluronic acid, Hyaluron, Hyaluronan EINECS/ELNICS: 232-678-0BIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 4. Sodium Hyaluronate - StructureSodium hyaluronate (sodium salt of hyaluronic acid) is alinear chain muco-polysaccharide composed ofdisaccharide units formed from D-glucuronic acid & N-acetylglucosamine held together by -1,3-linkages BIO/003/2012-13Dr. Lakshmi Sridhar18th Sep 2012 5. Sodium Hyaluronate Polymer produced by the growth of the bacteriumStreptococcus equi zooepidemicus; produced by bacterialfermentation; not of animal origin Non GMO; free of contaminants such as glycosaminoglycans &animal proteins Highly solubility; insoluble in water immiscible solvents Highly pure product; excellent transparency & stability Moisture retention; free radical scavenging, cell proliferation BIO/002/2012-13Dr. Lakshmi Sridhar18th Sep 2012 6. Process Flow DiagramBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 7. Sodium hyaluronate & the eye Hyaluronic acid significant constituent of human skin,connective tissue, vitreous humor of the eye, lubricant injoints, BIO/003/2012-13Dr. Lakshmi Sridhar18th Sep 2012 8. Sodium hyaluronate & the eye Hyaluronic acid is present in a high concentrationinside the eyeball.Vitreous humor inside the eye is almost completelymade up of hyaluronic acidThe hyaluronic acid has gel like properties whichgive shock absorbing effectTransports nutrients into the eye BIO/003/2012-13Dr. Lakshmi Sridhar18th Sep 2012 9. Sodium hyaluronate & the eyeBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 10. Sodium hyaluronate & the eyeOther ophthalmic applications of Sodiumhyaluronate Opthalmic drops for post eye surgery care Contact lens solutions Artificial tearsBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 11. Sodium hyaluronate With age, the hyaluronic acid in our joints decreases;leads to loss of cushioning & joint pains & aches. Injections with Hyaluronic acid & cross linkedhyaluronic acid gel give the joint lubrication &cushioning for load bearingBIO/002/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 12. Sodium hyaluronateIntraarticular injections to alleviate jointpains in senior citizens, sports persons,patients recovering from fractures of bonesBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 13. Sodium hyaluronate Tissue engineering & scaffolds & dermal fillers forcosmetic surgery Pharma grade hyaluronic acid is cross linked to forma gel Cross linked hyaluronic acid gels are more stable &get degraded more slowly by the enzymehyaluronidase than non cross linked hyaluronic acid The frequency of injections can be reducedBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012 14. Sodium HyaluronateBefore & after dermalDermal filler application to remove filler treatmentwrinkles & fill out naso-labial folds BIO/003/2012-13Dr. Lakshmi Sridhar18th Sep 2012 15. Sodium hyaluronateBiodegradable & eco-friendlySodium hyaluronate is completely naturalProduced by bacterial fermentationGreen technologyBIO/003/2012-13 Dr. Lakshmi Sridhar 18th Sep 2012