23 CpGs
1
23 CpGs TATA CAAT Enhancer core SP1 A EGFP SFFV B C 21 CpGs TATA CAAT EGFP EF1a S3: DNA methylation analysis on SFFV and EF1α promoters in P19 cells follow tivirus transduction. A: Methylation analysis on SFFV in P19 cells with 0.41 VCN shown in Figure S2a, at day 4 post-transduction, showing a 45 to 65% methylated CpGs from 6 of 12 (50%) analysed vector copies. B: Methylation analysis on SFFV in P19 cells with 0.41 VCN shown in Figure S2a, at day 14 post-transduction, showing a 65 to 100% methylated CpGs from 7 of 12 (58%) analysed vector copies. C: Methylation on EF1α in P19 cells with 0.38 VCN shown in Figure S2b, at day 14
description
EGFP. Figure S3: DNA methylation analysis on SFFV and EF1 α promoters in P19 cells following the lentivirus transduction. SFFV. SP1. Enhancer core. CAAT. TATA. 23 CpGs. A. B. EGFP. EF1a. CAAT. TATA. 21 CpGs. C. - PowerPoint PPT Presentation
Transcript of 23 CpGs
23 CpGs
TATACAATEnhancer coreSP1
A
EGFPSFFV
B
C21 CpGs
TATACAAT
EGFPEF1a
Figure S3: DNA methylation analysis on SFFV and EF1α promoters in P19 cells following the lentivirus transduction.
A: Methylation analysis on SFFV in P19 cells with 0.41 VCN shown in Figure S2a, at day 4post-transduction, showing a 45 to 65% methylated CpGs from 6 of 12 (50%) analysed vector copies.
B: Methylation analysis on SFFV in P19 cells with 0.41 VCN shown in Figure S2a, at day 14 post-transduction, showing a 65 to 100% methylated CpGs from 7 of 12 (58%) analysed vector copies.C: Methylation on EF1α in P19 cells with 0.38 VCN shown in Figure S2b, at day 14 post-transduction, showing a 40 to 100% methylated CpGs from 5 of 11(45%) analysed vector copies. Solid boxes. methylated CpGs
