100% Aqueous Compatible

INTRODUCING HALO® 90Å AQ-C18Introducing the new HALO® AQ-C18, a new bonded phase that is available on the Fused-Core® 2.7 μm superficially porous particle design with a 0.5 μm shell and 90Å pores. The AQ-C18 is a C18 bonded phase prepared using a proprietary procedure that increases phase polarity, making the AQ-C18 an excellent alternative C18 to consider, especially in aqueous mobile phases. HALO AQ-C18 is very complimentary to classic HALO C18 and extends C18 usefulness to 100% aqueous mobile phases.

Advantages of the New AQ-C18: • Resistant to dewetting and compatible with 100% aqueous mobile phases • Different selectivity than HALO C18, offering another option to resolve difficult peak pairs • Retains polar molecules more than classic C18 phases under most mobile phase conditions

Figure 1. The unique polar modified bonded phase of HALO AQ-C18 enables it to be run in 100% aqueous mobile phase without experiencing loss in retention due to dewetting when pressure is relieved. The retention is nearly 100% maintained compared to the HALO C18 after the pump is stopped and restarted.

RESISTANCE TO DEWETTING

TEST CONDITIONS:Column: 4.6 x 50 mm Top: HALO 90Å AQ-C18, 2.7 µm Bottom: HALO 90Å C18, 2.7 µmPart Numbers: Top: 92814-422 Bottom: 92814-402

Mobile Phase: 100% 20 mM Potassium Phosphate buffer, pH 7Flow Rate: 2 mL/minTemperature: 30 °C

Detection: 254 nmInjection: 0.5 µLSample: (1) thiourea, (2) 5-fluorocytosine, (3) adenine and (4) thymine

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Figure 3. The manufacturing process of HALO 90Å AQ-C18 is highly controlled, which results in a reproducible product as demonstrated by the lot-to-lot comparisons in Figure 3. The percent RSDs for the retention factors are all below 1.5% while the alpha values of adjacent peaks have percent RSDs lower than 1%.

Figure 4. Stability of the HALO 90Å AQ-C18 columns is demonstrated using pH 2 mobile phase conditions. The retention is maintained after 19,000 column volumes.

INCREASED RETENTION FOR POLAR COMPOUNDS — DIFFERENT SELECTIVITY

LOT-TO-LOT REPRODUCIBILITY

HIGH STABILITY AT LOW PH

TEST CONDITIONS:Column: 4.6 x 100 mm

Top: HALO 90Å AQ-C18, 2.7 µm

Bottom: HALO 90Å C18, 2.7 µm

Part Numbers: Top: 92814-622

Bottom: 92814-602

Mobile Phase A: Water

Mobile Phase B: Methanol

Isocratic: 50/50 A/B

Flow Rate: 1.3 mL/min

Temperature: 35 °C

Detection: 254 nm

Injection: 0.5 µL

Sample: (1) cinnamyl alcohol, (2) 4-bromoacetanilide, (3) nitrobenzene, (4) 3,4-dinitrotoluene, (5) anisole and (6) 2,4-dinitrotoluene

TEST CONDITIONS:Column: 4.6 x 100 mm

HALO 90Å AQ-C18, 2.7 µm

Part Number: 92814-622

Mobile Phase A: Water

Mobile Phase B: Methanol

Isocratic: 50/50 A/B

Flow Rate: 1.3 mL/min

Temperature: 35 °C

Detection: 254 nm

Injection: 0.5 µL

Sample: (1) cinnamyl alcohol, (2) 4-bromoacetanilide, (3) nitrobenzene, (4) 3,4-dinitrotoluene, (5) anisole and (6) 2,4-dinitrotoluene

TEST CONDITIONS:Column: 2.1 x 50 mm

HALO 90Å AQ-C18, 2.7 µm

Part Number: 92812-422

Mobile Phase A: 50 mM KCl/HCl, pH 2

Mobile Phase B: Acetonitrile

Isocratic: 60/40 A/B

Flow Rate: 1.0 mL/min

Pressure: 235 bar

Temperature: 60 °C

Detection: 254 nm

Injection: 0.5 µL

Sample: (1) amitriptyline and (2) naphthalene

Figure 2. The HALO AQ-C18 column exhibits increased retention of polar compounds along with a different elution order relative to the HALO C18.

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HALO AQ-C18

Dimension - ID x length (mm) Part No.

Dimension - ID x length (mm) Part No.

2.1 x 20 92812-222 3.0 x 100 92813-622

2.1 x 30 92812-322 3.0 x 150 92813-722

2.1 x 50 92812-422 3.0 x 250 92813-922

2.1 x 75 92812-522 4.6 x 20 92814-222

2.1 x 100 92812-622 4.6 x 30 92814-322

2.1 x 150 92812-722 4.6 x 50 92814-422

2.1 x 250 92812-922 4.6 x 75 92814-522

3.0 x 20 92813-222 4.6 x 100 92814-622

3.0 x 30 92813-322 4.6 x 150 92814-722

3.0 x 50 92813-422 4.6 x 250 92814-922

3.0 x 75 92813-522

HALO AQ-C18 Guard Columns, 3/Pack

Dimension - ID x length (mm) Part No.

2.1 x 5 92812-122

3.0 x 5 92813-122

4.6 x 5 92814-122

Guard Column Holder (1) 94900-001