DNA.

" [Cu(dppzdppe)]BF. DNA ". : . , .... (1111200900139) (1111200900128) University of AthensDepartment of Chemistry

: [Cu(Dppz)(Dppe)](BF4) DNA .

DNA. DNA , . , Watson Crick 1953 DNA . DNA .

DNA DNA . DNA . .

DNA. DNA .

Watson Crick , -DNA, DNA,

.

-DNA -DNA .

H cisplatin DNA () ( ), [Pt(NH3)2(H2O)2]2+. , . DNA-cisplatin DNA, .

Van der Waals

:

(intercalation) (groove binding) (external binding)

(INTERCALATION) DNA. 1961, o Lerman (intercalation). : DNA.

(GROOVE BINDING) (groove binder) - Van deer Waals .

-External Binding . DNA .

. ( Hg(II), Pb(II), Cd(II) . : Pt(II) Au(II) Cu(II) .

Cu(I)

dppz dppz . dppz, : , .

dppe dppe -1,2-() IUPAC .

NaPPh.

-.

>630 nm. .

: (HpD) . : : .

, , .

PTD .

350-1100nm.

21

: [Cu(CH3CN)4](BF4) Cu2O + HBF4 + CH3CN [Cu2O(CH3CN)4](BF4) DNA pR322

DNA : Ct-DNA

Tris-HCl(5mM) NaCl(50mM), pH=7,0 TBE 10x (Tris Borate EDTA) 100mL (Loading buffer)

[Cu(Dppz)(Dppe)](BF4) Cu(I). [Cu(MeCN)4](BF4),

[Cu(MeCN)4](BF4) : O2

[Cu(Dppz)(Dppe)](BF4) : - (UV-Vis) (NMR) (IR)

- (UV-Vis).

425 nm

MeCN = 0,49607*104 M cm-1

CH2Cl2 = 0,28092*104 M cm-1

(NMR).

dppedppzdppzdppzdppe

(IR).

C-C C-N BF4-

DNA : UV-Vis .

UV-Vis

b=2,7(1,3)104M-1 377nm.

:

DNA , :

, , .

Xe 1000W >335 nm.

(supercoiled) pBR322 DNA.

CT-DNA DNA. 2,7 (1,3)104 M-1 377nm (groove binding). , DNA , .