Αναγνωστοπούλου Ευαγγελία Ιανουάριος 2011
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Τμήμα Επιστήμης & Τεχνολογίας Υλικών ΒΙΟΟΡΓΑΝΙΚΕΣ ΝΑΝΟΔΟΜΕΣ. Αναγνωστοπούλου Ευαγγελία Ιανουάριος 2011. Self-Assembly of Coiled Coils in Synthetic Biology Hana Robson Marsden and Alexander Kros Angew. Chem. Int. Ed. 2010, 49, 2988 – 3005. Δομή των Coiled Coils Coiled Coils στη φύση - PowerPoint PPT Presentation
Transcript of Αναγνωστοπούλου Ευαγγελία Ιανουάριος 2011
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Self-Assembly of Coiled Coils in Synthetic Biology
Hana Robson Marsden and Alexander KrosAngew. Chem. Int. Ed. 2010, 49, 2988 3005
2011 & Coiled CoilsCoiled Coils
Coiled Coils
Bottom up ! A. Coiled Coil StructureAmphipathic = hydrophobic & lipolar
a-d : , b, c, f : e, g : Coiled coil =
Key role of Coiled coils : (2- 200 nm) - :
pH Coiled coils Short coiled coilsLong coiled coilsShort COILED COILS cellular Velcro ( 2-6 heptad )
complex
bZip (basic region leucine Zipper) (e,g) & DNA.
Long coiled coils , heptad .
long c-c< short c-c => long c-c :, & Spectrin protein , . - .
- & 2 spectrin .
B. COILED COILS IN NATURE - Dynamic functions & 1. INTRACELLULAR CABLES - myosinsMyosin F actin . coiled coil
TEM AFM of astretched muscle
L c-c =150nm
8
5.ADP
1. myosin actin.2. ATP.3. ATP myosin.4. ADP & Pi => .5. & actin.
2.ATP
3.
4.PiMyosin headActin filamentThick filament-+-+
2. Transport Vesicles snare proteins SNARE . 3 SNARE coiled coil SNAR. c-c Rab & GDP =>
Ph Mediated extension enveloped viruses
Enveloped viruses ( ) = . Influenza virus 3 coiled coil pH 7.4. sialic acid . - pH . 5 pH () .- . pH 7pH 5c. Synthetic coiled coils
PS = polystyrenePEG = poly(ethylene glycol)E & K = peptides E/K ? 3 heptad =>Short c-c=> .
- 1 : - 3 (hydrophilic peptides+hydrophobic polymer)2: 1. - 2. 3. F
: ( )
: , .
- coiled coils E/K PEGPSE/K PEGE/K PEGK PEGCoiled coil systems SNARE MODEL
SNARE modelSNARE : SNARE:1. E, K PEG. ( PEG: E, K .2. (DOPE) coiled-coil . v-SNARE.V-SNARESNAP-25t-SNARE
Amphipathic Short coiled coils: Velcro bZip (e,g) & DNA. Long coiled coils: long c-c< short c-cSpectrin protein . . Myosin (F ). / coiled coil. ATP ADP+Pi => actin. SNARE ( ). 3 SNARE coiled coil. GDP GTP => . pHInfluenza virus 3 coiled coil pH 7.4. 5 pH . Coiled Coils- c-c ~ . SNARE PEG (SNAP-25) & DOPE (v-SNARE).
Self-Assembly of Coiled Coils in Synthetic Biology: Inspiration and Progress, Hana Robson Marsden and Alexander KrosH. Robson Marsden, A. V. Korobko, E. N. M. van Leeuwen, E. M. Pouget, S. J. Veen, N. Sommerdijk, A. Kros, J. Am. Chem. Soc. 2008, 130, 9386.H. Robson Marsden, N. A. Elbers, P. H. H. Bomans, N. Sommerdijk, A. Kros, Angew. Chem. 2009, 121, 2366; Angew. Chem. Int. Ed. 2009, 48, 2330.
